The physical maps for sequencing human chromosomes 1, 6, 9, 10, 13, 20 and X

• Published in: Nature (London) Vol. 409; no. 6822; pp. 942 - 943
• Main Authors: Bentley, D. R, Deloukas, P, Dunham, A, French, L, Gregory, S. G, Humphray, S. J, Mungall, A. J, Ross, M. T, Carter, N. P, Dunham, I, Scott, C. E, Ashcroft, K. J, Atkinson, A. L, Aubin, K, Beare, D. M, Bethel, G, Brady, N, Brook, J. C, Burford, D. C, Burrill, W. D, Burrows, C, Butler, A. P, Carder, C, Catanese, J. J, Clee, C. M, Clegg, S. M, Cobley, V, Coffey, A. J, Cole, C. G, Collins, J. E, Conquer, J. S, Cooper, R. A, Culley, K. M, Dawson, E, Dearden, F. L, Durbin, R. M, de Jong, P. J, Dhami, P. D, Earthrowl, M. E, Edwards, C. A, Evans, R. S, Gillson, C. J, Ghori, J, Green, L, Gwilliam, R, Hammond, S, Harper, G. L, Heathcott, R. W, Holden, J. L, Holloway, E, Hopkins, B. L, Howard, P. J, Howell, G. R, Huckle, E. J, Hughes, J, Hunt, P. J, Hunt, S. E, Izmajlowicz, M, Jones, C. A, Joseph, S. S, Laird, G, Langford, C. F, Lehvaslaiho, M. H, Leversha, M. A, McCann, O. T, McDonald, L. M, McDowall, J, Maslen, G. L, Mistry, D, Moschonas, N. K, Neocleous, V, Pearson, D. M, Phillips, K. J, Porter, K. M, Prathalingam, S. R, Ramsey, Y. H, Ranby, S. A, Rice, C. M, Rogers, J, Rogers, L. J, Sarafidou, T, Scott, D. J, Sharp, G. J, Shaw-Smith, C. J, Smink, L. J, Soderlund, C, Sotheran, E. C, Steingruber, H. E, Sulston, J. E, Taylor, A, Taylor, R. G, Thorpe, A. A, Tinsley, E, Warry, G. L, Whittaker, A, Whittaker, P, Williams, S. H, Wilmer, T. E, Wooster, R, Wright, C. L
• Format: Journal Article
• Language: English
• Published: England Nature Publishing Group 15-02-2001
• Subjects: Bacteria; Chromosome 1; chromosome 10; chromosome 13; chromosome 20; chromosome 22; chromosome 6; chromosome 9; Chromosomes; Chromosomes, Human, Pair 1; Chromosomes, Human, Pair 10; Chromosomes, Human, Pair 13; Chromosomes, Human, Pair 20; Chromosomes, Human, Pair 6; Cloning; Containment; Contig Mapping; Gene mapping; Genes; Genome, Human; Genomics; Humans; X Chromosome
• ISSN: 0028-0836; 1476-4687
• DOI: 10.1038/35057165

We constructed maps for eight chromosomes (1, 6, 9, 10, 13, 20, X and (previously) 22), representing one-third of the genome, by building landmark maps, isolating bacterial clones and assembling contigs. By this approach, we could establish the long-range organization of the maps early in the project, and all contig extension, gap closure and problem-solving was simplified by containment within local regions. The maps currently represent more than 94% of the euchromatic (gene-containing) regions of these chromosomes in 176 contigs, and contain 96% of the chromosome-specific markers in the human gene map. By measuring the remaining gaps, we can assess chromosome length and coverage in sequenced clones.