Collectin CL-LK Is a Novel Soluble Pattern Recognition Receptor for Mycobacterium tuberculosis

• Published in: PloS one Vol. 10; no. 7; p. e0132692
• Main Authors: Troegeler, Anthony, Lugo-Villarino, Geanncarlo, Hansen, Søren, Rasolofo, Voahangy, Henriksen, Maiken Lumby, Mori, Kenichiro, Ohtani, Katsuki, Duval, Carine, Mercier, Ingrid, Bénard, Alan, Nigou, Jérome, Hudrisier, Denis, Wakamiya, Nobutaka, Neyrolles, Olivier
• Format: Journal Article
• Language: English
• Published: United States Public Library of Science 14-07-2015; Public Library of Science (PLoS)
• Subjects: Active control; Animals; Case-Control Studies; Collectins - blood; Collectins - deficiency; Collectins - genetics; Collectins - immunology; Cytokines; Dendritic cells; Diagnostic systems; Female; Health care; Humans; Immune response; Immune system; Immunology; In Vitro Techniques; Infections; Lectins; Life Sciences; Ligands; Lipopolysaccharides - immunology; Lipopolysaccharides - metabolism; Mannose; Mice; Mice, Inbred C57BL; Mice, Knockout; Mycobacterium tuberculosis; Mycobacterium tuberculosis - immunology; Mycobacterium tuberculosis - pathogenicity; Pattern recognition; Pattern recognition receptors; Receptors, Pattern Recognition - metabolism; Tuberculosis; Tuberculosis, Pulmonary - blood; Tuberculosis, Pulmonary - immunology
• ISSN: 1932-6203; 1932-6203
• DOI: 10.1371/journal.pone.0132692

Understanding the molecular components of immune recognition of the tuberculosis (TB) bacillus, Mycobacterium tuberculosis, can help designing novel strategies to combat TB. Here, we identify collectin CL-LK as a novel soluble C-type lectin able to bind M. tuberculosis, and characterize mycobacterial mannose-capped lipoarabinomannan as a primary ligand for CL-LK. Mice deficient in CL-K1, one of the CL-LK subunits, do not display altered susceptibility to M. tuberculosis. However, we found that the amount of CL-LK in the serum of patients with active TB is reduced, compared to that in controls, and correlates inversely to the magnitude of the immune response to the pathogen. These findings indicate that CL-LK might be of interest for future diagnostic and treatment monitoring purposes.