Enabling plant synthetic biology through genome engineering

• Published in: Trends in biotechnology (Regular ed.) Vol. 33; no. 2; pp. 120 - 131
• Main Authors: Baltes, Nicholas J, Voytas, Daniel F
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-02-2015; Elsevier Limited
• Subjects: Agrobacterium - genetics; Climate change; CRISPR/Cas; Deoxyribonucleic acid; Design; DNA; DNA End-Joining Repair; Endonucleases - genetics; Gene expression; Gene Expression Regulation, Plant; Gene Targeting - methods; Genetic engineering; Genetic Engineering - methods; genome engineering; Genome, Plant; Genomes; Genomics; Homologous Recombination; Internal Medicine; Metabolites; Methods; Mutagenesis, Site-Directed - methods; Nitrogen Fixation - genetics; Organisms; Plants - genetics; sequence-specific nuclease; Signal transduction; Synthetic biology; Synthetic Biology - methods; TALEN; Tobacco; Zinc Fingers - genetics; zinc-finger nuclease; synthetic biology; sequence-specific nuclease; zinc-finger nuclease; CRISPR/Cas; TALEN; genome engineering
• ISSN: 0167-7799; 1879-3096
• DOI: 10.1016/j.tibtech.2014.11.008

Highlights • Rewriting genomes will play an important role in plant synthetic biology. • Sequence-specific nucleases enable almost any DNA sequence change in plant cells. • The advantages and limitations of current sequence-specific nucleases are discussed. • A comprehensive list of recent plant genome engineering achievements is provided. • Achievements in genome engineering are related to plant synthetic biology projects.