Determination of Glyphosate in Water Samples by Multi-pumping Flow System Coupled to a Liquid Waveguide Capillary Cell

• Published in: Analytical Sciences Vol. 27; no. 10; p. 1031
• Main Authors: SILVA, Aline S., TÓTH, Ildikó V., PEZZA, Leonardo, PEZZA, Helena R., José L. F. C. LIMA
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2011; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: Analytical Chemistry; Chemistry; Flow Injection Analysis - instrumentation; Flow Injection Analysis - methods; Fresh Water - chemistry; Glycine - analogs & derivatives; Glycine - analysis; Glyphosate; Spectrophotometry, Ultraviolet; Water Pollutants, Chemical - analysis
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.27.1031

A simple screening method was developed for the determination of glyphosate in water samples using a multi-pumping flow system. The proposed method is based on the reaction between glyphosate and p-dimethylaminocinnamaldehyde (p-DAC), in an acid medium where the reaction product can be measured spectrophotometrically at λmax = 495 nm. An experimental design methodology was used to optimize the measurement conditions. The proposed method was applied to the determination of glyphosate in water samples in a concentration range from 0.5 to 10 μg mL−1. The limit of detection and quantification were 0.17 and 0.53 μg mL−1, respectively. The results obtained (88.5 to 104.5%) in recovery studies for the determination of glyphosate in different water samples indicated good accuracy and no matrix effect for the developed method. Samples were also analyzed by a confirmatory HPLC method, and agreement within the two set of results was found.