Neuroprotective effect of interleukin-6 regulation of voltage-gated Na＋ channels of cortical neurons is time- and dose-dependent

• Published in: Neural regeneration research Vol. 10; no. 4; pp. 610 - 617
• Main Authors: Xia, Wei, Peng, Guo-Yi, Sheng, Jiang-Tao, Zhu, Fang-Fang, Guo, Jing-Fang, Chen, Wei-Qiang
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01-04-2015; Medknow Publications & Media Pvt. Ltd; Department of Interventional Radiology, First Afifliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China%Department of Neurosurgery, First Afifliated Hospital, Shantou University Medical College, Shantou, Guangdong Province, China%Department of Pathogenic Microbiology and Immunology, Shantou University Medical College, Shantou, Guangdong Province, China; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Acids; action potential; adverse reactions; Alzheimer′s disease; AMPK; antiepileptic drugs; apoptosis; astrocytes; autografts; autophagy; biomaterials; blood flow; brain injury; CCN4; cell-adhesive ligands; central nervous system; cerebral infarction; cerebral ischemia; cerebrospinal fluid; co-culture; coma; compensation; cortical neurons; Cytokines; DDPH; diabetes mellitus; disability; dorsal root ganglia; dose-response curve; EGF; elastin-like proteins; EPO; erythropoietin; Experiments; extracellular matrix; FGF; fiber sprouting; Genetic aspects; glutamate receptor; health economics indicators; hippocampus; IGF-1; immature brain; in vitro experiments; infarct volume; inflammatory cytokines; inflammatory reaction; Interleukin-6; ischemic stroke; isolated basilar artery; L1CAM; long-term efficacy; matrix metalloproteinase; median nerve electrical stimulation; meta-analysis; molecular docking; mortality; mTOR; neonatal rats; nerve conduit; nerve graft; nerve growth factor; nerve regeneration; Nervous system; neural plasticity; neural regeneration; neural repair; neuroimmunomodulation; neurological function; neuron; Neurons; neuropathy; neurophysiology; neuroprotection; neurotrophic factors; NSFC grant; NSFC grants; orexin-A; OX1R; oxidative stress; patch clamp; peripheral nerve injury; Physiological aspects; Physiology; propriospinal detours; propriospinal system; psychiatric; puerarin; randomized controlled trial; recurrence; regeneration; Regulation; resveratrol; seizure; Sodium channels; spinal cord injury; stem cells; synaptic plasticity; systemic reviews; Transwell; traumatic brain injury; Tumor necrosis factor-TNF; vagus nerve stimulation; veins; voltage-gated Na + channel; wake-promoting; Wallerian degeneration; WISP1; Wnt; Xingnao Kaiqiao needling method; 剂量; 持续时间; 电压依赖性; 电压门控钠通道; 白细胞介素-6; 白细胞介素6; 皮质神经元; 神经保护作用; United States; United States > US; China; Grand Island New York; voltage-gated Na+ channel; patch clamp; action potential; interleukin-6; cortical neurons; brain injury; inflammatory reaction; neural regeneration; neuroimmunomodulation; neuroprotection; NSFC grants; cerebrospinal fluid; nerve regeneration; neurophysiology
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.155436

Interleukin-6 has been shown to be involved in nerve injury and nerve regeneration, but the effects of long-term administration of high concentrations of interleukin-6 on neurons in the central nervous system is poorly understood. This study investigated the effects of 24 hour expo-sure of interleukin-6 on cortical neurons at various concentrations （0.1, 1, 5 and 10 ng/mL） and the effects of 10 ng/mL interleukin-6 exposure to cortical neurons for various durations （2, 4, 8, 24 and 48 hours） by studying voltage-gated Na＋ channels using a patch-clamp technique. Volt-age-clamp recording results demonstrated that interleukin-6 suppressed Na＋ currents through its receptor in a time- and dose-dependent manner, but did not alter voltage-dependent activation and inactivation. Current-clamp recording results were consistent with voltage-clamp recording results. Interleukin-6 reduced the action potential amplitude of cortical neurons, but did not change the action potential threshold. The regulation of voltage-gated Na＋channels in rat corti-cal neurons by interleukin-6 is time- and dose-dependent.