Effect of hyperthermia on calbindin-D 28k immunoreactivity in the hippocampal formation following transient global cerebral ischemia in gerbils

• Published in: Neural regeneration research Vol. 12; no. 9; pp. 1458 - 1464
• Main Authors: Lee, Jae-Chul, Cho, Jeong-Hwi, Lee, Tae-Kyeong, Kim, In Hye, Won, Moo-Ho, Cho, Geum-Sil, Shin, Bich-Na, Hwang, In Koo, Park, Joon Ha, Ahn, Ji Hyeon, Kang, Il Jun, Lee, Young Joo, Kim, Yang Hee
• Format: Journal Article
• Language: English
• Published: India Medknow Publications and Media Pvt. Ltd 01-09-2017; Medknow Publications & Media Pvt. Ltd; Department of Neurobiology, School of Medicine, Kangwon National University, Chuncheon, South Korea%Department of Pharmacology & Toxicology, Shinpoong Pharmaceutical Co., Ltd., Ansan, South Korea%Department of Physiology, College of Medicine, Hallym University, Chuncheon, South Korea%Department of Anatomy and Cell Biology, College of Veterinary Medicine, and Research Institute for Veterinary Science, Seoul National University, Seoul, South Korea%Department of Biomedical Science, Research Institute of Bioscience and Biotechnology, Hallym University, Chuncheon, South Korea%Department of Food Science and Nutrition, Hallym University, Chuncheon, South Korea%Department of Emergency Medicine, Seoul Hospital, College of Medicine, Sooncheonhyang University, Seoul, South Korea%Department of Surgery, School of Medicine, Kangwon National University, Chuncheon, South Korea; Medknow Publications & Media Pvt Ltd; Wolters Kluwer Medknow Publications
• Subjects: Apoptosis; Body temperature; Brain; Calcium binding proteins; Carotid arteries; Cerebral ischemia; Complications and side effects; Development and progression; Fever; Health aspects; Hyperthermia; Ischemia; Laboratory animals; Morphology; nerve regeneration; hyperthermic condition; ischemia/reperfusion injury; subregions of hippocampus; delayed neuronal death; calbindin D-28k; neural regeneration; Neurons; Physiological aspects; Software; Variance analysis; 免疫作用; 免疫组织化学方法; 沙土鼠; 海马; 神经元损伤; 脑缺血; 钙结合蛋白; 高温条件; United States > US; Germany; delayed neuronal death; neural regeneration; ischemia/reperfusion injury; calbindin D-28k; nerve regeneration; hyperthermic condition; subregions of hippocampus
• ISSN: 1673-5374; 1876-7958
• DOI: 10.4103/1673-5374.215256

Calbindin D-28K （CB）, a Ca2＋-binding protein, maintains Ca2＋ homeostasis and protects neurons against various insults. Hyperthermia can exacerbate brain damage produced by ischemic insults. However, little is reported about the role of CB in the brain under hyperthermic condition during ischemic insults. We inves- tigated the effects of transient global cerebral ischemia on CB immunoreactivity as well as neuronal damage in the hippocampal formation under hyperthermic condition using immunohistochemistry for neuronal nuclei （NeuN） and CB, and Fluoro-Jade B histofluorescence staining in gerbils. Hyperthermia （39.5 ＋ 0.2~C） was induced for 30 minutes before and during transient ischemia. Hyperthermic ischemia resulted in neu- ronal damage/death in the pyramidal layer of CA1-3 area and in the polymorphic layer of the dentate gyrus at 1, 2, 5 days after ischemia. In addition, hyperthermic ischemia significantly decreaced CB immunoreac- tivity in damaged or dying neurons at 1, 2, 5 days after ischemia. In brief, hyperthermic condition produced more extensive and severer neuronal damage/death, and reduced CB immunoreactivity in the hippocampus following transient global cerebral ischemia. Present findings indicate that the degree of reduced CB immu- noreactivity might be related with various neuronal damage/death overtime and corresponding areas after ischemic insults.