distinct tospovirus causing necrotic streak on Alstroemeria sp. in Colombia

• Published in: Archives of virology Vol. 155; no. 3; pp. 423 - 428
• Main Authors: Hassani-Mehraban, Afshin, Botermans, Marleen, Verhoeven, J. Th. J, Meekes, Ellis, Saaijer, Janneke, Peters, Dick, Goldbach, Rob, Kormelink, Richard
• Format: Journal Article
• Language: English
• Published: Vienna Vienna : Springer Vienna 01-03-2010; Springer Vienna; Springer; Springer Nature B.V
• Subjects: Alstroemeria; Alstroemeria - virology; Biological and medical sciences; Biomedical and Life Sciences; Biomedicine; Brief Report; Cloning; Cloning, Molecular; Cluster Analysis; Colombia; Economic impact; Flowers & plants; Frankliniella occidentalis; Fundamental and applied biological sciences. Psychology; Groundnut ringspot virus; Infections; Infectious Diseases; Leaves; Lycopersicon esculentum; Medical Microbiology; Microbiology; Miscellaneous; Molecular Sequence Data; Phylogeny; Phytopathology. Animal pests. Plant and forest protection; Plant Diseases - virology; Plant viruses and viroids; Proteins; RNA, Viral - genetics; Sequence Analysis, DNA; Sequence Homology, Amino Acid; Serology; Serotyping; Tomato spotted wilt virus; Tospovirus; Tospovirus - classification; Tospovirus - genetics; Tospovirus - immunology; Tospovirus - isolation & purification; Viral Proteins - genetics; Virology; Viruses; South America; Colombia; America; United States > US; Netherlands; Western Flower Thrips; Tomato Spot Wilt Virus; Iris Yellow Spot Virus; Thrips Larva; Virus; Liliaceae; Monocotyledones; Bunyaviridae; Plant pathogen; Tospovirus; Angiospermae; Herbaceous plant; Spermatophyta
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-010-0590-7

A tospovirus causing necrotic streaks on leaves was isolated from Alstroemeria sp. in Colombia. Infected samples reacted positively with tomato spotted wilt virus (TSWV) antiserum during preliminary serological tests. Further analysis revealed a close serological relationship to tomato chlorotic spot virus (TCSV) and groundnut ringspot virus (GRSV). A major part of the S-RNA segment, encompassing the nucleocapsid (N) protein gene, the 5′ untranslated region and a part of the intergenic region 3′ of the N gene, was cloned and sequenced. The deduced N protein sequence showed highest amino acid identity (82%) to that of TCSV, indicating that the virus represents a new tospovirus species, for which the name Alstroemeria necrotic streak virus (ANSV) is coined. Phylogenetic analysis based on the N protein sequence revealed that this Alstroemeria-infecting tospovirus clustered with tospoviruses from the American continent. Frankliniella occidentalis was identified as potential vector species for ANSV.