SPE and HPLC/UV of resin acids in colophonium-containing products

• Published in: Journal of separation science Vol. 31; no. 15; pp. 2784 - 2790
• Main Authors: Nilsson, Ulrika, Berglund, Naghmeh, Lindahl, Fredrik, Axelsson, Sara, Redeby, Theres, Lassen, Pia, Karlberg, Ann-Therese
• Format: Journal Article
• Language: English
• Published: Weinheim Wiley-VCH Verlag 01-08-2008; WILEY-VCH Verlag; WILEY‐VCH Verlag; Wiley
• Subjects: Abietane; Abscisic Acid; Abscisic Acid - analysis; Allergic contact dermatitis; analysis; Analytical chemistry; Applied sciences; Arbets- och miljömedicin; Biological and medical sciences; Chemical industry and chemicals; Chemical Sciences; chemical synthesis; Chemistry; Chromatographic methods and physical methods associated with chromatography; Chromatography; Chromatography, High Pressure Liquid - instrumentation; Chromatography, High Pressure Liquid - methods; Colophonium; Cosmetics, toiletries; Diterpenes; Diterpenes, Abietane - analysis; Diterpenes, Abietane - chemical synthesis; Ethanolamines; Ethanolamines - analysis; Exact sciences and technology; Folkhälsomedicinska forskningsområden; General aspects; High Pressure Liquid; HPLC/UV; Immunopathology; instrumentation; Kemi; Medical sciences; MEDICIN; MEDICINE; methods; Molecular Conformation; Occupational and Environmental Medicine; Occupational medicine; Other chromatographic methods; Plant; Public health medicine research areas; Resin acids; Resins; Resins, Plant - analysis; Resins, Plant - chemistry; Sensitivity and Specificity; Social medicine; Socialmedicin; Solid Phase Extraction; Solid Phase Extraction - instrumentation; Solid Phase Extraction - methods; SPE; Spectrophotometry; Spectrophotometry, Ultraviolet - instrumentation; Spectrophotometry, Ultraviolet - methods; Stereoisomerism; Ultraviolet; Washing products. Cosmetics and toiletries. Perfumes; Yrkesmedicin; Trace analysis; Cosmetic; Solid phase extraction; Terpenoid; Chemical analysis; Allergic contact dermatitis; HPLC chromatography; SPE; Chemical enrichment; Makeup product; Ultraviolet detector; Sample preparation; Natural gum; Colophonium HPLC/UV; Diterpene; Diode array; Resin acids; Foundation; Mixed adsorbent; Depilatory; Resinic acid; Allergen; Quantitative analysis
• ISSN: 1615-9306; 1615-9314; 1615-9314
• DOI: 10.1002/jssc.200800210

A new method, involving SPE and HPLC/UV diode-array detection (DAD), was developed for the quantification of colophonium components in different consumer products, such as cosmetics. Colophonium is a common cause of contact dermatitis since its components can oxidize into allergens on exposure to air. Three different resin acids were used as markers for native and oxidized colophonium, abietic acid (AbA), dehydroabietic acid (DeA), and 7-oxodehydroabietic acid (7-O-DeA). The SPE method, utilizing a mixed-mode hydrophobic and anion exchange retention mechanism, was shown to yield very clean extracts. The use of a urea-embedded C₁₂ HPLC stationary phase improved the separation of the resin acids compared to common C₁₈. Concentrations higher than 2 mg/g of both AbA and DeA were detected in wax strips. In this product also 7-O-DeA, a marker for oxidized colophonium, was detected at a level of 28 μg/g. The LODs were in the range of 7-19 μg/g and the LOQs 22-56 μg/g. The method is simple to use and can be applied on many types of technical products, not only cosmetics. For the first time, a method for technical products was developed, which separates AbA from pimaric acid.