Lactobacilli with superoxide dismutase-like or catalase activity are more effective in alleviating inflammation in an inflammatory bowel disease mouse model

• Published in: Drug design, development and therapy Vol. 12; pp. 3221 - 3233
• Main Authors: Tomusiak-Plebanek, Anna, Heczko, Piotr, Skowron, Beata, Baranowska, Agnieszka, Okoń, Krzysztof, Thor, Piotr J, Strus, Magdalena
• Format: Journal Article
• Language: English
• Published: New Zealand Dove Medical Press Limited 01-01-2018; Taylor & Francis Ltd; Dove Medical Press
• Subjects: Abscess; Abscesses; Analysis; Animals; antioxidative enzymes; Bacteria; Body mass; Body Mass Index; Body temperature; Catalase; Catalase - metabolism; Colon; Colon - metabolism; Colon - microbiology; Colon - pathology; Colorectal cancer; Cytokines; Disease Models, Animal; Enzymes; Gastrointestinal diseases; Hydrogen peroxide; Inflammation; Inflammation - metabolism; Inflammation - microbiology; Inflammation - pathology; Inflammation - therapy; Inflammatory bowel disease; Inflammatory bowel diseases; Inflammatory Bowel Diseases - metabolism; Inflammatory Bowel Diseases - microbiology; Inflammatory Bowel Diseases - pathology; Inflammatory Bowel Diseases - therapy; Interleukin-10 - deficiency; Interleukin-10 - genetics; Interleukin-10 - metabolism; Intestine; Intestines; Lactobacilli; Lactobacillus; Lactobacillus - enzymology; Lactobacillus - isolation & purification; Lactobacillus - metabolism; Medical research; Methods; Mice; Mice, Inbred C57BL; Mice, Knockout; Microbiology; Microbiota; mouse model; Original Research; Oxidation; probiotic strains; Probiotics; Researchers; Superoxide dismutase; Superoxide Dismutase - metabolism; Superoxides; Poland; United States > US; inflammatory bowel diseases; mouse model; probiotic strains; antioxidative enzymes
• ISSN: 1177-8881; 1177-8881
• DOI: 10.2147/DDDT.S164559

Some lactobacilli, which possess superoxide dismutase-like activity and catalase activity naturally, have strong antioxidative properties. The aim of this study was to identify such strains and check which of them play a crucial role in alleviating intestinal inflammation.
We selected two
strains for use in animal studies:
30B (which has the highest catalase activity) and
900 (which has the highest dismutase-like activity). Forty mice (C57B1/6J) were divided into four experimental groups with ten mice in each group. Group I (control group) was not supplemented with
, group II (catalase group) was orally supplemented with
30B, group III (dismutase-like group) was supplemented with
900, and group IV (mixed group) was supplemented with both
strains. For 23 days, the temperature and body mass of each mouse were recorded and fecal samples for microbiological examination were collected. On day 23, the animals were sacrificed, and their intestines were removed for microbiological and histopathological studies.
Compared to the control group, the highest drop in the body temperature was observed in groups II (
<0.05) and IV (
<0.05). Similarly, groups II (
<0.05) and IV (
<0.05) had the highest drop in body mass. Moreover, histopathological evaluation of colon fragments showed intracryptic abscesses in these groups. Group III mice showed most limited degree of inflammation.
strains with dismutase-like activity are more effective in alleviating intestinal inflammation than strains producing catalase, suggesting that superoxide anion radical decomposition is crucial in this process.