Antibacterial activity of Bacillus species-derived surfactin on Brachyspira hyodysenteriae and Clostridium perfringens

• Published in: AMB Express Vol. 9; no. 1; pp. 1 - 9
• Main Authors: Horng, Yi-Bing, Yu, Yu-Hsiang, Dybus, Andrzej, Hsiao, Felix Shih-Hsiang, Cheng, Yeong-Hsiang
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Springer Berlin Heidelberg 21-11-2019; Springer Nature B.V; SpringerOpen
• Subjects: Animal husbandry; Antibacterial activity; Antibacterial agents; Antibiotics; Bacillus; Bacillus licheniformis; Bacteria; Biomedical and Life Sciences; Biotechnology; Brachyspira; Brachyspira hyodysenteriae; Clostridium perfringens; Dogs; Dysentery; Enteritis; Fermented food; Food additives; Growth promoters; Isoforms; Life Sciences; Microbial Genetics and Genomics; Microbiology; Morphology; Original; Original Article; Species; Surfactin; Swine; Swine dysentery; Antibacterial activity; Surfactin
• ISSN: 2191-0855; 2191-0855
• DOI: 10.1186/s13568-019-0914-2

Swine dysentery and necrotic enteritis are a bane to animal husbandry worldwide. Some countries have already banned the use of antibiotics as growth promoters in animal production. Surfactin is a potential alternative to antibiotics and antibacterial agents. However, the antibacterial activity of
Bacillus
species-derived surfactin on
Brachyspira hyodysenteriae
and
Clostridium perfringens
are still poorly understood. In the current study, the antibacterial effects of surfactin produced from
Bacillus subtilis
and
Bacillus licheniformis
on
B. hyodysenteriae
and
C. perfringens
were evaluated. Results showed that multiple surfactin isoforms were detected in
B. subtilis
, while only one surfactin isoform was detected in
B. licheniformis
fermented products. The surfactin produced from
B. subtilis
exhibited significant antibacterial activity against
B. hyodysenteriae
compared with surfactin produced from
B. licheniformis
.
B. subtilis
-derived surfactin could inhibit bacterial growth and disrupt the morphology of
B. hyodysenteriae
. Furthermore, the surfactin produced from
B. subtilis
have the highest activity against
C. perfringens
growth. In contrast,
B. licheniformis
fermented product-derived surfactin had a strong bacterial killing activity against
C. perfringens
compared with surfactin produced from
B. subtilis
. These results together suggest that
Bacillus
species-derived surfactin have potential for development as feed additives and use as a possible substitute for antibiotics to prevent
B. hyodysenteriae
and
C. perfringens
-associated disease in the animal industry.