Development and characterization of a new rice cultivar, ‘Chikushi-kona 85’, derived from a starch-branching enzyme IIb-deficient mutant line

A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding. ‘Chikushi-kona 85’ showed a higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’, and a higher grain y...

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Published in:	Breeding Science Vol. 68; no. 2; pp. 278 - 283
Main Authors:	Wada, Takuya, Yamaguchi, Osamu, Miyazaki, Masayuki, Miyahara, Katsunori, Ishibashi, Masafumi, Aihara, Takeshi, Shibuta, Takanobu, Inoue, Takashi, Tsubone, Masao, Toyosawa, Yoshiko, Satoh, Hikaru, Akaishi, Ryuuichirou, Yoshii, Youichi, Ohtsubo, Ken’ichi
Format:	Journal Article
Language:	English
Published:	Japan Japanese Society of Breeding 01-03-2018 Japan Science and Technology Agency
Subjects:	Amylopectin Breeding control of blood sugar level Cookies Crop yield Deficient mutant Enzymes Genetic analysis Grain Oryza Oryza sativa L resistant starch Rice Starch Starch-branching enzymes Sugar super-hard rice Oryza sativa L control of blood sugar level super-hard rice resistant starch
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Abstract	A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding. ‘Chikushi-kona 85’ showed a higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’, and a higher grain yield than the first super-hard rice cultivar, ‘EM10’. The amylopectin chain length of ‘Chikushi-kona 85’ and its progenitor line ‘EM129’ was longer than that of ‘Nishihomare’, and was similar to that of ‘EM10’. This suggests that the starch property of ‘Chikushi-kona 85’ was inherited from ‘EM129’, which is a mutant line deficient in a starch branching enzyme similar to ‘EM10’. Genetic analysis of ‘Chikushi-kona 85’ crossed with ‘Nishihomare’ also showed that the starch property of ‘Chikushi-kona 85’ was regulated by a single recessive gene. Consumption of processed cookies made from ‘Chikushi-kona 85’ flour showed a distinctive effect in controlling blood sugar levels in comparison to the normal non-glutinous rice cultivar ‘Hinohikari’. These results show that ‘Chikushi-kona 85’ is a novel genetic source to develop new products made of rice, which could reduce calorie intake and contribute to additional health benefits.
AbstractList	A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding. ‘Chikushi-kona 85’ showed a higher content of resistant starch than the normal non-glutinous rice cultivar, ‘Nishihomare’, and a higher grain yield than the first super-hard rice cultivar, ‘EM10’. The amylopectin chain length of ‘Chikushi-kona 85’ and its progenitor line ‘EM129’ was longer than that of ‘Nishihomare’, and was similar to that of ‘EM10’. This suggests that the starch property of ‘Chikushi-kona 85’ was inherited from ‘EM129’, which is a mutant line deficient in a starch branching enzyme similar to ‘EM10’. Genetic analysis of ‘Chikushi-kona 85’ crossed with ‘Nishihomare’ also showed that the starch property of ‘Chikushi-kona 85’ was regulated by a single recessive gene. Consumption of processed cookies made from ‘Chikushi-kona 85’ flour showed a distinctive effect in controlling blood sugar levels in comparison to the normal non-glutinous rice cultivar ‘Hinohikari’. These results show that ‘Chikushi-kona 85’ is a novel genetic source to develop new products made of rice, which could reduce calorie intake and contribute to additional health benefits.
Author	Miyazaki, Masayuki Ohtsubo, Ken’ichi Toyosawa, Yoshiko Akaishi, Ryuuichirou Shibuta, Takanobu Yamaguchi, Osamu Satoh, Hikaru Miyahara, Katsunori Ishibashi, Masafumi Tsubone, Masao Aihara, Takeshi Wada, Takuya Inoue, Takashi Yoshii, Youichi
AuthorAffiliation	5 Institute of Genetic Resources, Faculty of Agriculture, Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan 1 Fukuoka Agriculture and Forestry Research Center , 587 Yoshiki, Chikushino, Fukuoka 818-8549 , Japan 3 Department of Research and Development, Torigoe Company Limited , 6-8-8 HakozakiFuto, Higashi-ku, Fukuoka 812-0051 , Japan 6 Niigata Agricultural Research Institute , 2-25 Shin’ei-cho, Kamo-shi, Niigata 959-1381 , Japan 7 Faculty of Agriculture, Niigata University , 8050 Ikarashi-ninocho, Nishi-ku, Niigata 950-2181 , Japan
AuthorAffiliation_xml	– name: 6 Niigata Agricultural Research Institute , 2-25 Shin’ei-cho, Kamo-shi, Niigata 959-1381 , Japan – name: 3 Department of Research and Development, Torigoe Company Limited , 6-8-8 HakozakiFuto, Higashi-ku, Fukuoka 812-0051 , Japan – name: 1 Fukuoka Agriculture and Forestry Research Center , 587 Yoshiki, Chikushino, Fukuoka 818-8549 , Japan – name: 5 Institute of Genetic Resources, Faculty of Agriculture, Kyushu University , 6-10-1 Hakozaki, Higashi-ku, Fukuoka 812-8581 , Japan – name: 7 Faculty of Agriculture, Niigata University , 8050 Ikarashi-ninocho, Nishi-ku, Niigata 950-2181 , Japan
Author_xml	– sequence: 1 fullname: Wada, Takuya organization: Fukuoka Agriculture and Forestry Research Center – sequence: 2 fullname: Yamaguchi, Osamu organization: Fukuoka Agriculture and Forestry Research Center – sequence: 3 fullname: Miyazaki, Masayuki organization: Fukuoka Agriculture and Forestry Research Center – sequence: 4 fullname: Miyahara, Katsunori organization: Fukuoka Agriculture and Forestry Research Center – sequence: 5 fullname: Ishibashi, Masafumi organization: Fukuoka Agriculture and Forestry Research Center – sequence: 6 fullname: Aihara, Takeshi organization: Department of Research and Development, Torigoe Company Limited – sequence: 7 fullname: Shibuta, Takanobu organization: Department of Research and Development, Torigoe Company Limited – sequence: 8 fullname: Inoue, Takashi organization: Fukuoka Agriculture and Forestry Research Center – sequence: 9 fullname: Tsubone, Masao organization: Fukuoka Agriculture and Forestry Research Center – sequence: 10 fullname: Toyosawa, Yoshiko organization: Institute of Genetic Resources, Faculty of Agriculture, Kyushu University – sequence: 11 fullname: Satoh, Hikaru organization: Institute of Genetic Resources, Faculty of Agriculture, Kyushu University – sequence: 12 fullname: Akaishi, Ryuuichirou organization: Niigata Agricultural Research Institute – sequence: 13 fullname: Yoshii, Youichi organization: Niigata Agricultural Research Institute – sequence: 14 fullname: Ohtsubo, Ken’ichi organization: Faculty of Agriculture, Niigata University
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Copyright	2018 by JAPANESE SOCIETY OF BREEDING Copyright Japan Science and Technology Agency 2018 Copyright © 2018 by JAPANESE SOCIETY OF BREEDING 2018
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Notes	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Present address: Faculty of Applied Life Sciences, Niigata University of Pharmacy and Applied Life Sciences, 265-1 Higashijima, Akiha-ku, Niigata 956-8603, Japan Present address: Fukuoka Prefectural Office, 7-7 Higashi-koen, Hakata-ku, Fukuoka 812-8577, Japan Communicated by Hiroshi Kato Present address: Kitakyushu Agricultural Extension Center of Fukuoka Prefecture, 3-7-1 Norimatsu, Yahata-nishi, Kitakyushu, Fukuoka 807-0831, Japan
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Snippet	A new super-hard rice cultivar, ‘Chikushi-kona 85’, which was derived from a cross between ‘Fukei 2032’ and ‘EM129’, was developed via bulk method breeding.... A new super-hard rice cultivar, 'Chikushi-kona 85', which was derived from a cross between 'Fukei 2032' and 'EM129', was developed via bulk method breeding....
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SubjectTerms	Amylopectin Breeding control of blood sugar level Cookies Crop yield Deficient mutant Enzymes Genetic analysis Grain Oryza Oryza sativa L resistant starch Rice Starch Starch-branching enzymes Sugar super-hard rice
Title	Development and characterization of a new rice cultivar, ‘Chikushi-kona 85’, derived from a starch-branching enzyme IIb-deficient mutant line
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