Characterization of Insulin Adsorption Behavior on Amphoteric Charged Membranes

• Published in: Polymer journal Vol. 40; no. 9; pp. 837 - 841
• Main Authors: Zhang, Shaoling, Saito, Keiichiro, Matsumoto, Hidetoshi, Minagawa, Mie, Tanioka, Akihiko
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 01-09-2008; Society of Polymer Science; Nature Publishing Group
• Subjects: Adsorption; Amphoterics; Applied sciences; Biological and medical sciences; Biomaterials; Bioorganic Chemistry; Charging; Chemistry; Chemistry and Materials Science; Chemistry/Food Science; Exact sciences and technology; Exchange resins and membranes; Forms of application and semi-finished materials; General pharmacology; Insulin; Medical sciences; Membranes; Pharmaceutical technology. Pharmaceutical industry; Pharmacology. Drug treatments; Polymer industry, paints, wood; Polymer Sciences; regular-article; Surface charge; Surface chemistry; Surfaces and Interfaces; Technology of polymers; Thin Films; Zeta potential; Zeta Potential; Adsorption; Insulin; Poly(acrylonitrile); Amphoteric Charged Membrane; Acrylic acid copolymer; Polyampholyte; Liquid solid adsorption; Control release polymer; Adsorption capacity; Drug carrier; Acrylamide derivative copolymer; Experimental study; Bigraft copolymer; Acrylonitrile copolymer; pH; Membrane; Electrokinetic potential; Kinetics; Amine copolymer
• ISSN: 0032-3896; 1349-0540
• DOI: 10.1295/polymj.PJ2008093

With view to develop an amphoteric charged membrane for drug delivery, we have studied the adsorption of insulin on it. In the present study, the amphoteric charged membranes were prepared by pore-surface modification of porous poly(acrylonitrile) (PAN) membranes by grafting with acrylic acid (AAc) and/or
N
,
N
-(dimethylamino)propyl acrylamide (DMAPAA). Their surface charge properties and the insulin adsorption behaviors were investigated by zeta potential measurement and UV spectrophotoscopy, respectively, at different pHs. The equilibrium adsorbed amount of insulin correlates well with the charge properties of the membranes and insulin, which indicates that electrostatic interaction played an important role in the insulin adsorption. In addition, adsorption kinetics changed from a Fickian mode to a non-Fickian one when adsorbed amount increased to very high values.