Bone Formation on Murine Cranial Bone by Injectable Cross-Linked Hyaluronic Acid Containing Nano-Hydroxyapatite and Bone Morphogenetic Protein

• Published in: Polymers Vol. 14; no. 24; p. 5368
• Main Authors: Hachinohe, Yuki, Taira, Masayuki, Hoshi, Miki, Hatakeyama, Wataru, Sawada, Tomofumi, Kondo, Hisatomo
• Format: Journal Article
• Language: English
• Published: Switzerland MDPI AG 08-12-2022; MDPI
• Subjects: Analysis; Animal experimentation; Biocompatibility; Biomedical materials; bone augmentation; bone morphogenetic protein; Bone morphogenetic proteins; Bone surgery; Bones; Calcium phosphates; Composite materials; Connective tissue; Connective tissues; cross-linked hyaluronic acid; Crosslinking; ectopic bone formation; Experiments; Fourier transforms; Growth factors; Hyaluronic acid; Hydrogels; Hydroxyapatite; In vivo methods and tests; injection-type bone forming material; Microscopy; nano hydroxyapatite; Polyethylene glycol; Polymers; Polytetrafluoroethylene; Proteins; Sol-gel processes; Spectrum analysis; X ray analysis; Japan; United States > US; cross-linked hyaluronic acid; injection-type bone forming material; bone morphogenetic protein; nano hydroxyapatite; bone augmentation; ectopic bone formation
• ISSN: 2073-4360; 2073-4360
• DOI: 10.3390/polym14245368

New injection-type bone-forming materials are desired in dental implantology. In this study, we added nano-hydroxyapatite (nHAp) and bone morphogenetic protein (BMP) to cross-linkable thiol-modified hyaluronic acid (tHyA) and evaluated its usefulness as an osteoinductive injectable material using an animal model. The sol (ux-tHyA) was changed to a gel (x-tHyA) by mixing with a cross-linker. We prepared two sol−gel (SG) material series, that is, x-tHyA + BMP with and without nHAp (SG I) and x-tHyA + nHAp with and without BMP (SG II). SG I materials in the sol stage were injected into the cranial subcutaneous connective tissues of mice, followed by in vivo gelation, while SG II materials gelled in Teflon rings were surgically placed directly on the cranial bones of rats. The animals were sacrificed 8 weeks after implantation, followed by X-ray analysis and histological examination. The results revealed that bone formation occurred at a high rate (>70%), mainly as ectopic bone in the SG I tests in mouse cranial connective tissues, and largely as bone augmentation in rat cranial bones in the SG II experiments when x-tHyA contained both nHAp and BMP. The prepared x-tHyA + nHAp + BMP SG material can be used as an injection-type osteoinductive bone-forming material. Sub-periosteum injection was expected.