Intranasal vaccination with recombinant receptor-binding domain of MERS-CoV spike protein induces much stronger local mucosal immune responses than subcutaneous immunization: Implication for designing novel mucosal MERS vaccines

Highlights • We compared MERS-CoV RBD-induced immune responses via i.n. and s.c. routes. • I.n. induced comparable systemic humoral immune responses as s.c. • I.n. induced similar neutralization but more robust cellular responses than s.c. • I.n. elicited significantly higher local mucosal immune re...

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Bibliographic Details
Published in:	Vaccine Vol. 32; no. 18; pp. 2100 - 2108
Main Authors:	Ma, Cuiqing, Li, Ye, Wang, Lili, Zhao, Guangyu, Tao, Xinrong, Tseng, Chien-Te K, Zhou, Yusen, Du, Lanying, Jiang, Shibo
Format:	Journal Article
Language:	English
Published:	Kidlington Elsevier Ltd 11-04-2014 Elsevier Elsevier Limited
Subjects:	Administration, Intranasal Allergy and Immunology Animals Antibodies, Neutralizing - blood Antibodies, Viral - blood Applied microbiology Biological and medical sciences Coronavirus Coronavirus - classification Coronavirus - immunology Female Fundamental and applied biological sciences. Psychology HEK293 Cells Humans Immune system Immunity, Cellular Immunity, Humoral Immunity, Mucosal Immunization Immunoglobulin Fc Fragments - immunology Immunoglobulin G - blood Injections, Subcutaneous Lung - immunology Medical research MERS-CoV Mice Mice, Inbred BALB C Microbiology Mucosal immune response Neutralizing antibody Pandemics Receptor-binding domain Recombinant Proteins - immunology Respiratory tract Rodents Spike Glycoprotein, Coronavirus - immunology Spike protein Spleen - immunology Systemic immune response Vaccines Vaccines, antisera, therapeutical immunoglobulins and monoclonal antibodies (general aspects) Viral infections Viral Vaccines - immunology Systemic immune response MERS-CoV Spike protein Receptor-binding domain Mucosal immune response Neutralizing antibody Immunization Immune response Mucosa Vaccination Vaccine Intranasal administration Protein
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Summary:	Highlights • We compared MERS-CoV RBD-induced immune responses via i.n. and s.c. routes. • I.n. induced comparable systemic humoral immune responses as s.c. • I.n. induced similar neutralization but more robust cellular responses than s.c. • I.n. elicited significantly higher local mucosal immune responses than s.c. • Has potential to be developed as an effective and safe MERS mucosal vaccine.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 ObjectType-Article-2 ObjectType-Feature-1 These authors contributed equally to this work.
ISSN:	0264-410X 1873-2518
DOI:	10.1016/j.vaccine.2014.02.004