Molecular identification of 1-Cys peroxiredoxin and anthocyanidin/flavonol 3-O-galactosyltransferase from proanthocyanidin-rich young fruits of persimmon (Diospyros kaki Thunb.)

• Published in: Planta Vol. 230; no. 4; pp. 841 - 855
• Main Authors: Ikegami, Ayako, Akagi, Takashi, Potter, Daniel, Yamada, Masahiko, Sato, Akihiko, Yonemori, Keizo, Kitajima, Akira, Inoue, Kentaro
• Format: Journal Article
• Language: English
• Published: Berlin/Heidelberg Berlin/Heidelberg : Springer-Verlag 01-09-2009; Springer-Verlag; Springer; Springer Nature B.V
• Subjects: Accumulation; Agriculture; Amino Acid Sequence; Amino acids; Anthocyanins - chemistry; Anthocyanins - metabolism; Biocatalysis; Biological and medical sciences; Biomedical and Life Sciences; Biosynthesis; Clone Cells; Complementary DNA; Developmental stages; Diospyros - enzymology; Diospyros - genetics; Diospyros - growth & development; E coli; Ecology; Enzymes; Flavonoids; Flavonols; Flavonols - chemistry; Flavonols - metabolism; Forestry; Fruit - enzymology; Fruit - growth & development; Fruits; Fundamental and applied biological sciences. Psychology; Galactosyltransferases - chemistry; Galactosyltransferases - metabolism; Gene Expression Regulation, Plant; Glycosylation; Libraries; Life Sciences; Molecular Sequence Data; Nucleic Acid Hybridization; Original; Original Article; Peroxiredoxins - chemistry; Peroxiredoxins - metabolism; Phylogeny; Plant Sciences; Plants; Proanthocyanidins - metabolism; Proteins; Reproducibility of Results; Reverse Transcriptase Polymerase Chain Reaction; RNA, Messenger - genetics; RNA, Messenger - metabolism; Sequence Analysis, Protein; Sequence Homology, Amino Acid; Flavonoid 3; 1-Cys peroxiredoxin; Proanthocyanidin; Persimmon; galactosyltransferase; Fruits; Diospyros kaki; Flavonoid 3-O-galactosyltransferase O Fruits; Fruit; Enzyme; Transferases; Glycosyltransferases; Plant biology; Flavonoid; N-Acetyllactosaminide α-1,3-galactosyltransferase; Molecular parameter; Ebenaceae; Dicotyledones; Angiospermae; Anthocyanidin; Hexosyltransferases; Spermatophyta; Fruit tree; Flavone(3-hydroxy)
• ISSN: 0032-0935; 1432-2048
• DOI: 10.1007/s00425-009-0989-0

Fruits of persimmon (Diospyros kaki Thunb.) accumulate large amounts of proanthocyanidins (PAs) in the early stages of development. Astringent (A)-type fruits remain rich in soluble PAs even after they reach full-mature stage, whereas non-astringent (NA)-type fruits lose these compounds before full maturation. As a first step to elucidate the mechanism of PA accumulation in this non-model species, we used suppression subtractive hybridization to identify transcripts accumulating differently in young fruits of A- and NA-type. Interestingly, only a few clones involved in PA biosynthesis were identified in A-NA libraries. Represented by multiple clones were those encoding a novel 1-Cys peroxiredoxin and a new member of family 1 glycosyltransferases. Quantitative RT-PCR analyses confirmed correlation of the amount of PAs and accumulation of transcripts encoding these proteins in young persimmon fruits. Furthermore, the new family 1 glycosyltransferase was produced in Escherichia coli and shown to efficiently catalyze galactosylation at 3-hydroxyl groups of several anthocyanidins and flavonols. These findings suggest a complex mechanism of PA accumulation in persimmon fruits.