Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system

Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond...

Full description

Saved in:

Bibliographic Details
Published in:	Acta crystallographica. Section F, Structural biology and crystallization communications Vol. 63; no. 9; pp. 734 - 736
Main Authors:	Hiraki, Toshiki, Shibayama, Naoya, Yoon, Young-Ho, Yun, Kyung-Mook, Hamamoto, Toshiro, Tame, Jeremy R. H., Park, Sam-Yong
Format:	Journal Article
Language:	English
Published:	5 Abbey Square, Chester, Cheshire CH1 2HU, England Blackwell Publishing Ltd 01-09-2007 International Union of Crystallography
Subjects:	Animals Baculoviridae Baculovirus Base Sequence Bombyx - enzymology Bombyx mori Carboxylesterase - chemistry Carboxylesterase - isolation & purification CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY CRYSTALLIZATION Crystallization Communications Crystallography, X-Ray CRYSTALS DIMERS DNA Primers esterase A4 Female Glycoproteins - chemistry Glycoproteins - isolation & purification Glycosylation Metalloproteins - chemistry Metalloproteins - isolation & purification Molecular Sequence Data Ovum - enzymology PROTEINS Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification RESOLUTION SOLVENTS SPACE GROUPS Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization SYNCHROTRON RADIATION WEIGHT
Online Access:	Get full text
Tags:	Add Tag No Tags, Be the first to tag this record!

Abstract	Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P21, with unit‐cell parameters a = 47.1, b = 73.9, c = 47.4 Å, β = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.3 Å3 Da−1 and the solvent content is 47%.
AbstractList	Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P2{sub 1}, with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 Å, β = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (V{sub M}) is 2.3 Å{sup 3} Da{sup −1} and the solvent content is 47%. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 A resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P2(1), with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 A, beta = 104.1 degrees. With one dimer per asymmetric unit, the crystal volume per unit protein weight (V(M)) is 2.3 A3 Da(-1) and the solvent content is 47%. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori . The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori . The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P 2 1 , with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 Å, β = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight ( V M ) is 2.3 Å 3 Da −1 and the solvent content is 47%. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 A resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P2 sub(1), with unit-cell parameters a = 47.1, b = 73.9, c = 47.4 A, beta = 104.1 degree . With one dimer per asymmetric unit, the crystal volume per unit protein weight (V sub(M)) is 2.3 A super(3) Da super(-1) and the solvent content is 47%. Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and expressed using a baculovirus expression system in silkworm pupae. Crystals of the purified protein have been grown that diffract to beyond 2.1 Å resolution at 100 K using synchrotron radiation. The protein crystals belong to space group P21, with unit‐cell parameters a = 47.1, b = 73.9, c = 47.4 Å, β = 104.1°. With one dimer per asymmetric unit, the crystal volume per unit protein weight (VM) is 2.3 Å3 Da−1 and the solvent content is 47%.
Author	Yoon, Young-Ho Shibayama, Naoya Hamamoto, Toshiro Yun, Kyung-Mook Tame, Jeremy R. H. Hiraki, Toshiki Park, Sam-Yong
Author_xml	– sequence: 1 givenname: Toshiki surname: Hiraki fullname: Hiraki, Toshiki – sequence: 2 givenname: Naoya surname: Shibayama fullname: Shibayama, Naoya – sequence: 3 givenname: Young-Ho surname: Yoon fullname: Yoon, Young-Ho – sequence: 4 givenname: Kyung-Mook surname: Yun fullname: Yun, Kyung-Mook – sequence: 5 givenname: Toshiro surname: Hamamoto fullname: Hamamoto, Toshiro – sequence: 6 givenname: Jeremy R. H. surname: Tame fullname: Tame, Jeremy R. H. – sequence: 7 givenname: Sam-Yong surname: Park fullname: Park, Sam-Yong
BackLink	https://www.ncbi.nlm.nih.gov/pubmed/17768340$$D View this record in MEDLINE/PubMed https://www.osti.gov/biblio/22360541$$D View this record in Osti.gov
BookMark	eNqFUktv1DAQjlAr-oAfwAVZQuK2YMeJnVyQVitaqFZF4qEKLtbEmewaEju1k9Llzv_GUValiENPHs18jxnPnCQH1llMkmeMvmKMytefmMwyTssYU86LPHuUHE-pxZQ7uBcfJSchfKcRVIricXLEpBQFz-hx8nvld2GAtjW_YDDOErA16T22pjMW_I7ofd1tPPRbo0kYxtpgIK4hwxZJh3O13WnXezegsQTDgB4CkmVGxmDshgCpQI-tuzF-DARvo0MIk12I6tg9SQ4baAM-3b-nyZezt59X7xbrD-fvV8v1QgtGs4UogCHnDcs0p01JqwrrVLIckGsqGQotoAYKskCIAWtqUTUMyow3CJpLfpq8mXX7seqw1mgHD63qvenirMqBUf9WrNmqjbtRKZeCszIKvJgFXBiMCtoMqLfaWYt6UGnKBc0zFlEv9zbeXY_xN1Rngsa2BYtuDEoUadwKLx8ERseCpWkWgWwGau9C8Njc9cyomm5B_XcLkfP8_rB_GfvlR0A5A36aFncPK6rl17N0vc4Zm8QXM9fE_d3eccH_UEJymaury3N1IcTVRco_qm_8DyWe1dM
CitedBy_id	crossref_primary_10_1016_j_jmb_2008_01_020
ContentType	Journal Article
Copyright	International Union of Crystallography 2007 2007
Copyright_xml	– notice: International Union of Crystallography 2007 2007
DBID	BSCLL CGR CUY CVF ECM EIF NPM AAYXX CITATION 7SS 7X8 OTOTI 5PM
DOI	10.1107/S1744309107033854
DatabaseName	Istex Medline MEDLINE MEDLINE (Ovid) MEDLINE MEDLINE PubMed CrossRef Entomology Abstracts (Full archive) MEDLINE - Academic OSTI.GOV PubMed Central (Full Participant titles)
DatabaseTitle	MEDLINE Medline Complete MEDLINE with Full Text PubMed MEDLINE (Ovid) CrossRef Entomology Abstracts MEDLINE - Academic
DatabaseTitleList	MEDLINE MEDLINE - Academic Entomology Abstracts
Database_xml	– sequence: 1 dbid: ECM name: MEDLINE url: https://search.ebscohost.com/login.aspx?direct=true&db=cmedm&site=ehost-live sourceTypes: Index Database
DeliveryMethod	fulltext_linktorsrc
Discipline	Engineering Chemistry
DocumentTitleAlternate	Esterase A4
EISSN	1744-3091
EndPage	736
ExternalDocumentID	22360541 10_1107_S1744309107033854 17768340 AYF2LL5114 ark_67375_WNG_J66WJ23R_Z
Genre	miscellaneous Research Support, Non-U.S. Gov't Journal Article
GroupedDBID	--- .3N .GA .Y3 05W 10A 1OC 23M 2WC 31~ 33P 4.4 50Y 50Z 51W 51X 52M 52N 52O 52P 52S 52T 52W 52X 53G 5GY 5HH 5LA 66C 6J9 702 7PT 8-0 8-1 8-3 8-4 8-5 8UM 930 A03 AAEVG AAHHS AANLZ AAONW AAXRX AAZKR ABCQN ABCUV ABEML ABJNI ACAHQ ACBWZ ACCFJ ACCZN ACGFS ACPOU ACSCC ACXBN ACXQS ADBBV ADEOM ADIYS ADIZJ ADMGS ADOZA ADZOD AEEZP AEIGN AEIMD AEQDE AEUQT AEUYR AFBPY AFEBI AFFPM AFGKR AFPWT AFZJQ AHBTC AITYG AIURR AIWBW AJBDE ALAGY ALMA_UNASSIGNED_HOLDINGS ALUQN AMBMR AMYDB AOIJS ASPBG ATUGU AVWKF AZBYB AZFZN BAWUL BDRZF BROTX BRXPI BSCLL BY8 CAG COF CS3 D-E D-F DCZOG DIK DPXWK DR2 DRFUL DRSTM EBS EJD EMB EMOBN F00 F01 F04 F5P FEDTE G-S G.N GODZA HGLYW HH5 HVGLF HYE HZI HZ~ I-F IX1 LATKE LC2 LC3 LEEKS LH4 LITHE LOXES LP6 LP7 LUTES LW6 LYRES MK4 MRFUL MRSTM MSFUL MSSTM MXFUL MXSTM N04 N05 N9A NF~ O9- OIG OK1 P2X P4D Q.N Q11 QB0 R.K RCJ RNS ROL RPM RX1 SUPJJ SV3 TR2 UB1 V8K W8V WIH WIK WOQ WQJ WRC XG1 ~IA ~WT CGR CUY CVF ECM EIF NPM AAMNL AAYXX CITATION 7SS 7X8 0R~ AAPBV ABHUG ABPTK ACXME ADAWD ADDAD AFVGU AGJLS OTOTI 5PM
ID	FETCH-LOGICAL-c6104-68a1e33f14c30f90bbed2715ae3c071e6c6ada0a78eaada1fd6bf1a943feac373
IEDL.DBID	RPM
ISSN	1744-3091
IngestDate	Tue Sep 17 21:27:32 EDT 2024 Fri May 19 01:42:56 EDT 2023 Sat Aug 17 05:30:04 EDT 2024 Fri Aug 16 20:37:07 EDT 2024 Thu Nov 21 21:51:06 EST 2024 Sat Nov 02 12:22:11 EDT 2024 Sat Aug 24 00:51:49 EDT 2024 Wed Oct 30 09:51:41 EDT 2024
IsDoiOpenAccess	false
IsOpenAccess	true
IsPeerReviewed	true
IsScholarly	true
Issue	9
Language	English
LinkModel	DirectLink
MergedId	FETCHMERGED-LOGICAL-c6104-68a1e33f14c30f90bbed2715ae3c071e6c6ada0a78eaada1fd6bf1a943feac373
Notes	ark:/67375/WNG-J66WJ23R-Z istex:3949C7D4BCCF5336349C639F36AFE0D840E277C5 ArticleID:AYF2LL5114 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
OpenAccessLink	https://europepmc.org/articles/pmc2376319?pdf=render
PMID	17768340
PQID	19381224
PQPubID	23462
PageCount	3
ParticipantIDs	pubmedcentral_primary_oai_pubmedcentral_nih_gov_2376319 osti_scitechconnect_22360541 proquest_miscellaneous_68230939 proquest_miscellaneous_19381224 crossref_primary_10_1107_S1744309107033854 pubmed_primary_17768340 wiley_primary_10_1107_S1744309107033854_AYF2LL5114 istex_primary_ark_67375_WNG_J66WJ23R_Z
PublicationCentury	2000
PublicationDate	September 2007
PublicationDateYYYYMMDD	2007-09-01
PublicationDate_xml	– month: 09 year: 2007 text: September 2007
PublicationDecade	2000
PublicationPlace	5 Abbey Square, Chester, Cheshire CH1 2HU, England
PublicationPlace_xml	– name: 5 Abbey Square, Chester, Cheshire CH1 2HU, England – name: England – name: United Kingdom
PublicationTitle	Acta crystallographica. Section F, Structural biology and crystallization communications
PublicationTitleAlternate	Acta Cryst. F
PublicationYear	2007
Publisher	Blackwell Publishing Ltd International Union of Crystallography
Publisher_xml	– name: Blackwell Publishing Ltd – name: International Union of Crystallography
SSID	ssj0033968
Score	1.6944116
Snippet	Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori. The gene for this metalloglycoprotein was cloned from B. mori eggs and... Esterase A4 (EA4) is a timer protein found in diapause eggs of the silkworm Bombyx mori . The gene for this metalloglycoprotein was cloned from B. mori eggs...
SourceID	pubmedcentral osti proquest crossref pubmed wiley istex
SourceType	Open Access Repository Aggregation Database Index Database Publisher
StartPage	734
SubjectTerms	Animals Baculoviridae Baculovirus Base Sequence Bombyx - enzymology Bombyx mori Carboxylesterase - chemistry Carboxylesterase - isolation & purification CONDENSED MATTER PHYSICS, SUPERCONDUCTIVITY AND SUPERFLUIDITY CRYSTALLIZATION Crystallization Communications Crystallography, X-Ray CRYSTALS DIMERS DNA Primers esterase A4 Female Glycoproteins - chemistry Glycoproteins - isolation & purification Glycosylation Metalloproteins - chemistry Metalloproteins - isolation & purification Molecular Sequence Data Ovum - enzymology PROTEINS Recombinant Proteins - chemistry Recombinant Proteins - isolation & purification RESOLUTION SOLVENTS SPACE GROUPS Spectrometry, Mass, Matrix-Assisted Laser Desorption-Ionization SYNCHROTRON RADIATION WEIGHT
Title	Crystallization and preliminary crystallographic studies of the metalloglycoprotein esterase A4 using a baculovirus expression system
URI	https://api.istex.fr/ark:/67375/WNG-J66WJ23R-Z/fulltext.pdf https://onlinelibrary.wiley.com/doi/abs/10.1107%2FS1744309107033854 https://www.ncbi.nlm.nih.gov/pubmed/17768340 https://search.proquest.com/docview/19381224 https://search.proquest.com/docview/68230939 https://www.osti.gov/biblio/22360541 https://pubmed.ncbi.nlm.nih.gov/PMC2376319
Volume	63
hasFullText	1
inHoldings	1
isFullTextHit
isPrint
link	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1Bb9MwGP1ExwE4IBgwMsbwAXFAyprEbpwcq7IyxpgQAw24WI5jQ7U0qdIFrT-A_81nJxmdBkLibCuJ8l7s98XPzwDPtY44zxXzYxqlPtMq85PQSJ_FJpMyN6HSdnPywQk__py82rcxOaN-L4wz7atstlcW871y9t15KxdzNex9YsP37ybWyYHUGQ5ggNqwL9Hb4ZfSNE665UssbYYnKLkZtbMicpsmI3cQD0eRTe3_jrW56KZ9rRc4Mlf4bf1Jb163Ta7LWTcfTe_B3U5IknH7wPfhhi434dakP79tE-6sRQ0-gJ-TeoVCsCi6fZdEljlZ1Lpwp3rVK6K69jbCeqbIsnUYksoQFIlkrtvWYqUqF-4wK4lLWcBpkIwZsQb6b0SSTKqmqH7M6mZJ9EVnsy1Jmxj9ED5N9z9ODvzuCAZfoa5CBBMZakpNyBQNTBpkmc4jHo6kpgrFiY5VLHMZSJ5oRFaGJo8zE8qUUYMjOuX0EWyUVakfAwk5VVQrG5EXMKkUXjnNEMYsCQyyg3nwsgdDLNqkDeEqlICLayB68MLBddlT1mfWosZH4vT4tTiM49PDiH4QXz3YsXgK1BM2FFdZ95A6FyiKsI5joQfPepwFwmMXS2Spq2YpUNgmdtXx7z1iu0aZ0tSDrZYXvx-745cH_ApjLjvYTO-rLUh1l-3dUduDyHHr329CjL9Mo6Mj1M1s-79v9wRut_-srXduBzbO60Y_hcEyb3axzHjzdtd9Yr8AAnMsgQ
link.rule.ids	230,315,729,782,786,887,27933,27934,53800,53802
linkProvider	National Library of Medicine
linkToHtml	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwnV1db9MwFL1i28PggY_xFRjMD4gHpKxJ7MbJY1VWyugqxIYGe7Ecx4GKNKnSBa0_gP_NtZOMTgMh7dlWPnyPfY_t42OAV1oHnKeKuSENYpdplbiRn0mXhVkiZZr5SpvDyeNjPv0SvT0wNjn97iyMFe2rZLZf5PP9YvbdaisXc9XrdGK9j0dDo-RA6PQ2YAv7q-d1k_RmAKY0DqN2AxMnN71jJN2MmryI6KZR317Fw5FmU7PisZaNtkzDXuDYXGLv-hvjvC6cXCe0NiON7t3wX-7D3ZaCkkFT_ABu6WIHtofdzW87cGfNpPAh_BpWK6SQed6e2CSySMmi0rm9D6xaEdWWN-bXM0WWjTaRlBlBeknmuinNV6q0thCzglh_BkygZMCIkd5_I5IkUtV5-XNW1UuiL1qBbkEar-lH8Hl0cDIcu-3lDa5CRoaxj6SvKc18pqiXxV6S6DTgfl9qqpDW6FCFMpWe5JFGTEg_S8Mk82XMaIa5gHL6GDaLstBPgficKqqVMdfzmFQKnxwn2GhJ5GWIK-bAmy6IYtF4dAg7t_G4uBZ8B17bMF_WlNUPI27jfXE6fScOw_D0MKCfxJkDuwYHApmIsdNVRnekzgXSKZwBMt-BvQ4fAsNjtllkoct6KZASR2a_8t81QrO7GdPYgScNnv58dotLB_gVpF1WMG7gV0sQWNYVvAWSA4HF5P9bQgy-joLJBBk3e3bj1-3B9vjkaCIm76cfnsPtZuXbKPB2YfO8qvUL2Fim9UvbQX8DyABBDw
linkToPdf	http://sdu.summon.serialssolutions.com/2.0.0/link/0/eLvHCXMwpV1bb9MwGP3ENonLA5dxCwzmB8QDUtYkduPksepWxijVxEBjvFiOY0NFmlTpitYfwP_ms5OWTgMhwbOtXOxjf8f28fkAXmgdcZ4r5sc0Sn2mVeYnoZE-i00mZW5Cpe3l5MMTPvqU7B9Ym5xVqi8n2lfZeK8sJnvl-KvTVk4nqrPUiXWO3_WtkgOh05nmprMBWzhmg2i5UG8mYUrTOGkPMXGB0zlB4s2ojY2IcJp0XToejlSb2l2PtYi0ZRv3AufnCkfY71jnVfHkOql1UWlw5z_-5y7cbqko6TVV7sE1XW7Djf4yA9w23FozK7wPP_r1AqlkUbQ3N4ksczKtdeHygtULotryxgR7rMis0SiSyhCkmWSim9JioSpnDzEuifNpwEBKeoxYCf4XIkkm1byovo_r-Yzoi1aoW5LGc_oBfBwcfOgf-m0SB18hM0MMJDLUlJqQKRqYNMgynUc87EpNFdIbHatY5jKQPNGIDRmaPM5MKFNGDcYEyulD2CyrUj8GEnKqqFbWZC9gUil8cpphw2VJYBBfzINXy44U08arQ7g1TsDFFQB48NJ19aqmrL9ZkRvvitPRa3EUx6dHEX0vPnuwY7EgkJFYW11l9UfqXCCtwpUgCz3YXWJEYPfY4xZZ6mo-E0iNE3tu-ecasT3lTGnqwaMGU78-u8WmB_wS2lYVrCv45RIEl3MHb8HkQeRw-feWEL2zQTQcIvNmT_75dbtw_Xh_IIZvRm-fws1mA9wK8XZg87ye62ewMcvnz90Y_QnB9kOP
openUrl	ctx_ver=Z39.88-2004&ctx_enc=info%3Aofi%2Fenc%3AUTF-8&rfr_id=info%3Asid%2Fsummon.serialssolutions.com&rft_val_fmt=info%3Aofi%2Ffmt%3Akev%3Amtx%3Ajournal&rft.genre=article&rft.atitle=Crystallization+and+preliminary+crystallographic+studies+of+the+metalloglycoprotein+esterase+A4+using+a+baculovirus+expression+system&rft.jtitle=Acta+crystallographica.+Section+F%2C+Structural+biology+and+crystallization+communications&rft.au=Hiraki%2C+Toshiki&rft.au=Shibayama%2C+Naoya&rft.au=Yoon%2C+Young%E2%80%90Ho&rft.au=Yun%2C+Kyung%E2%80%90Mook&rft.date=2007-09-01&rft.pub=Blackwell+Publishing+Ltd&rft.issn=1744-3091&rft.eissn=1744-3091&rft.volume=63&rft.issue=9&rft.spage=734&rft.epage=736&rft_id=info:doi/10.1107%2FS1744309107033854&rft.externalDBID=10.1107%252FS1744309107033854&rft.externalDocID=AYF2LL5114
thumbnail_l	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/lc.gif&issn=1744-3091&client=summon
thumbnail_m	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/mc.gif&issn=1744-3091&client=summon
thumbnail_s	http://covers-cdn.summon.serialssolutions.com/index.aspx?isbn=/sc.gif&issn=1744-3091&client=summon