High resolution DNA barcode library for European butterflies reveals continental patterns of mitochondrial genetic diversity

• Published in: Communications biology Vol. 4; no. 1; p. 315
• Main Authors: Dincă, Vlad, Dapporto, Leonardo, Somervuo, Panu, Vodă, Raluca, Cuvelier, Sylvain, Gascoigne-Pees, Martin, Huemer, Peter, Mutanen, Marko, Hebert, Paul D. N., Vila, Roger
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 09-03-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 45/22; 45/23; 45/77; 631/208; 631/208/514/1948; 631/601/1466; 706/648; Animals; Biodiversity; Biology; Biomedical and Life Sciences; Butterflies - classification; Butterflies - genetics; Conservation; Datasets; Deoxyribonucleic acid; DNA; DNA barcoding; DNA Barcoding, Taxonomic; DNA, Mitochondrial - genetics; Europe; Evolution; Gene Library; Genetic diversity; Genetic Variation; Haplotypes; Life Sciences; Mitochondria; Refugia; Species; Species Specificity; Taxonomy; Europe
• ISSN: 2399-3642; 2399-3642
• DOI: 10.1038/s42003-021-01834-7

The study of global biodiversity will greatly benefit from access to comprehensive DNA barcode libraries at continental scale, but such datasets are still very rare. Here, we assemble the first high-resolution reference library for European butterflies that provides 97% taxon coverage (459 species) and 22,306
COI
sequences. We estimate that we captured 62% of the total haplotype diversity and show that most species possess a few very common haplotypes and many rare ones. Specimens in the dataset have an average 95.3% probability of being correctly identified. Mitochondrial diversity displayed elevated haplotype richness in southern European refugia, establishing the generality of this key biogeographic pattern for an entire taxonomic group. Fifteen percent of the species are involved in barcode sharing, but two thirds of these cases may reflect the need for further taxonomic research. This dataset provides a unique resource for conservation and for studying evolutionary processes, cryptic species, phylogeography, and ecology.
Vlad Dincă, Leonardo Dapporto, and colleagues provide a continental-scale DNA barcode library for 97% of the 473 European butterfly species and examine patterns of haplotype diversity. Results of mitochondrial diversity show elevated haplotype richness in southern European refugia, and this dataset provides a valuable resource for specimen identification, conservation, and research.