Induced rates of mitotic crossing over and possible mitotic gene conversion per wing anlage cell in Drosophila melanogaster by X rays and fission neutrons

• Published in: Genetics (Austin) Vol. 126; no. 1; pp. 157 - 166
• Main Authors: Ayaki, T. (Nagasaki University School of Medicine, Nagasaki, Japan), Fujikawa, K, Ryo, H, Itoh, T, Kondo, S
• Format: Journal Article
• Language: English
• Published: Bethesda, MD Genetics Soc America 01-09-1990; Genetics Society of America
• Subjects: 560152 - Radiation Effects on Animals- Animals; ABERRACION CROMOSOMICA; ABERRATION CHROMOSOMIQUE; ADN; AILE; ALAS; ANIMALS; ARTHROPODS; BARYONS; Biological and medical sciences; BIOLOGICAL EFFECTS; BIOLOGICAL RADIATION EFFECTS; BIOLOGICAL RECOVERY; BIOLOGICAL REPAIR; CELL DIVISION; CHROMOSOMAL ABERRATIONS; Chromosome Aberrations; Classical genetics, quantitative genetics, hybrids; CROSSING OVER; Crossing Over, Genetic; CRUZAMIENTO INTERCROMOSOMICO; DIPTERA; DNA Damage; DNA REPAIR; DROSOPHILA; DROSOPHILA MELANOGASTER; Drosophila melanogaster - anatomy & histology; Drosophila melanogaster - genetics; Drosophila melanogaster - radiation effects; Drosophilidae; ELECTROMAGNETIC RADIATION; ELEMENTARY PARTICLES; EUMYCOTA; FERMIONS; FISSION NEUTRONS; FLIES; FRUIT FLIES; Fundamental and applied biological sciences. Psychology; FUNGI; GENE; Gene Conversion; GENE MUTATIONS; GENE RECOMBINATION; GENES; GENETIC EFFECTS; GENETIC RADIATION EFFECTS; Genetics of eukaryotes. Biological and molecular evolution; HADRONS; Humans; INSECTS; Invertebrata; INVERTEBRATES; Investigations; IONIZING RADIATIONS; MICROORGANISMS; MITOSE; MITOSIS; Models, Genetic; MUTATIONS; NEUTRONS; NUCLEONS; PLANTS; RADIACIONES; RADIATION; RADIATION EFFECTS; RADIATION, THERMAL, AND OTHER ENVIRON. POLLUTANT EFFECTS ON LIVING ORGS. AND BIOL. MAT; RADIATIONS; RADIOINDUCTION; RAYON X; RAYOS-X; RBE; RECOMBINACION; RECOMBINAISON; Recombination, Genetic; RECOVERY; REPAIR; STRAND BREAKS; X RADIATION; YEASTS; Yeasts - genetics; Chromosomal aberration; Recombination; Mitosis; Insecta; X ray irradiation; Drosophilidae; Arthropoda; DNA; Neutron irradiation; Invertebrata; Gene conversion; Crossing over; Repair; Drosophila melanogaster; Diptera
• DOI: 10.1093/genetics/126.1.157
• EISSN: 1943-2631
• ISSN: 0016-6731; 1943-2631

As a model for chromosome aberrations, radiation-induced mitotic recombination of mwh and flr genes in Drosophila melanogaster strain (mwh +/+ flr) was quantitatively studied. Fission neutrons were five to six times more effective than X rays per unit dose in producing either crossover-mwh/flr twins and mwh singles-or flr singles, indicating that common processes are involved in the production of crossover and flr singles. The X-ray-induced rate/wing anlage cell/Gy for flr singles was 1 x 10-5, whereas that of crossover was 2 x 1O-4; the former and the latter rate are of the same order of magnitude as those of gene conversion and crossover in yeast, respectively. Thus, we conclude that proximal-marker "flr" singles induced in the transheterozygote are gene convertants. Using the model based on yeast that recombination events result from repair of double-strand breaks or gaps, we propose that mitotic recombination in the fly is a secondary result of recombinational DNA repair. Evidence for recombinational misrepair in the fly is given. The relative ratio of radiation-induced mitotic crossover to spontaneous meiotic crossover is one order of magnitude higher in the fly than in yeast and humans