Decellularized extracellular matrix microparticles as a vehicle for cellular delivery in a model of anastomosis healing

• Published in: Journal of biomedical materials research. Part A Vol. 104; no. 7; pp. 1728 - 1735
• Main Authors: Hoganson, David M., Owens, Gwen E., Meppelink, Amanda M., Bassett, Erik K., Bowley, Chris M., Hinkel, Cameron J., Finkelstein, Eric B., Goldman, Scott M., Vacanti, Joseph P.
• Format: Journal Article
• Language: English
• Published: United States Blackwell Publishing Ltd 01-07-2016; Wiley Subscription Services, Inc
• Subjects: 3-D cell culture; Anastomosis; Anastomosis, Surgical; Animals; Automotive components; Binding sites; Bioreactors; cell adhesion; cell migration; Cell Movement; Cellular; Collagen; Drug Delivery Systems - methods; Electrochemical machining; Exposure; Extracellular matrix; Extracellular Matrix - metabolism; Fibroblasts; Fibroblasts - cytology; Gravitation; Gravity; Healing; Humans; In vitro methods and tests; In vitro testing; Microparticles; Microspheres; Models, Biological; Paracrine signalling; Peritoneum; Repair; Surface area; Surgical implants; Sus scrofa; Tensile Strength; Therapy; Viability; Wound Healing; cell migration; microparticles; cell adhesion; extracellular matrix; 3-D cell culture
• ISSN: 1549-3296; 1552-4965
• DOI: 10.1002/jbm.a.35703

Extracellular matrix (ECM) materials from animal and human sources have become important materials for soft tissue repair. Microparticles of ECM materials have increased surface area and exposed binding sites compared to sheet materials. Decellularized porcine peritoneum was mechanically dissociated into 200 µm microparticles, seeded with fibroblasts and cultured in a low gravity rotating bioreactor. The cells avidly attached and maintained excellent viability on the microparticles. When the seeded microparticles were placed in a collagen gel, the cells quickly migrated off the microparticles and through the gel. Cells from seeded microparticles migrated to and across an in vitro anastomosis model, increasing the tensile strength of the model. Cell seeded microparticles of ECM material have potential for paracrine and cellular delivery therapies when delivered in a gel carrier. © 2016 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 104A: 1728–1735, 2016.