The potential role of migratory birds in the transmission of pathogenic Campylobacter species to broiler chickens in broiler poultry farms and live bird markets

• Published in: BMC microbiology Vol. 23; no. 1; p. 66
• Main Authors: Tawakol, Maram M, Nabil, Nehal M, Samir, Abdelhafez, M, Hawash H, Yonis, Ahlam E, Shahein, Momtaz A, Elsayed, Mona M
• Format: Journal Article
• Language: English
• Published: England BioMed Central Ltd 10-03-2023; BioMed Central; BMC
• Subjects: Amikacin; Animals; Anti-Bacterial Agents; Antibiotic resistance; Antibiotics; Antimicrobial agents; Biosecurity; Bird impact; Bird migration; Broiler chicken; Broilers (Poultry); Campylobacter; Campylobacter coli - genetics; Campylobacter infections; Campylobacter Infections - microbiology; Campylobacter jejuni - genetics; Campylobacter spp; Chickens; Chickens - microbiology; Diseases; Distribution; Doxycycline; Drinking water; Drug resistance; Farms; Food contamination & poisoning; Genes; Health hazards; Humans; Infections; Influence; Migratory birds; Migratory species; Multiantibiotic resistance; Multidrug resistance; Poultry; Poultry - microbiology; Poultry Diseases - microbiology; Poultry farming; Poultry farms; Public health; Strains (organisms); Virulence; Egypt; Europe; Africa; Migratory birds; Poultry farms; Campylobacter spp; Broiler chicken
• ISSN: 1471-2180; 1471-2180
• DOI: 10.1186/s12866-023-02794-0

Campylobacter species (spp.) are one of the most important zoonotic bacteria possessing potential hazards for animal and human health worldwide. Migratory birds are implicated as significant carriers for microbes and a play very important role in the dissemination of Campylobacter to broiler chickens and their environment. The purpose of this investigation was to detect the prevalence, antibiotic resistant patterns, virulence and diversity of pathogenic Campylobacter spp. in 7 migratory bird species (Northern shoveler, Common pochard, Common teal, Northern pintail, Eared Grebe, Great Crested Grebe and Garganey) and broiler chickens that were collected from broiler poultry farms and live bird markets.
The prevalence of Campylobacter was 12.5% (25/200), of which 15% (15/100) was recovered from 5 migratory bird species only and 10% (10/100) from broiler chickens. At the level of migratory birds, eight isolates (53.3%) were Campylobacter jejuni (C. jejuni) and 7 isolates (46.7%) were Campylobacter coli (C. coli) meanwhile, in broiler chickens C. jejuni and C. coli were 50% (5/10) for each. All isolated strains had phenotypic resistance to doxycycline, while all of the isolates were susceptible to amikacin. The multidrug resistance to three, four or five antimicrobial classes was found in 72% (18/25) of the isolated strains. The multiantibiotic resistance index between the examined isolates was 0.22-0.77, with 10 antibiotic resistance patterns. The virulence of isolated Campylobacter strains (from both migratory birds and broiler chicken birds) was detected by targeting the VirB11, ciaB and iam genes which were recorded at 16%, 52% and 100%, respectively. Additionally, 100% and 84% of the antibiotic resistance genes were identified as tetA and BlaOXA-61, respectively.
The results of this study revealed the diversity between all the isolated strains from migratory birds and their similarity to broiler chicken isolates. The findings of the present study highlight the impact of migratory birds visiting Egypt and other countries on pathogenic Campylobacter spp. carrying pathogenic virulence and resistance genes, necessitating the application of biosecurity measures to prevent migratory birds from entering farms during their migration period.