Collection, Focusing, and Metering of DNA in Microchannels Using Addressable Electrode Arrays for Portable Low-Power Bioanalysis

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 103; no. 13; pp. 4825 - 4830
• Main Authors: Shaikh, Faisal A., Ugaz, Victor M.
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 28-03-2006; National Acad Sciences
• Subjects: Anodes; Biomolecules; Biosensors; Deoxyribonucleic acid; DNA; DNA - analysis; DNA - genetics; DNA - isolation & purification; Electric fields; Electrodes; Electrophoresis; Engineering; Equipment Design; Gels; Glass; Hydrodynamics; Kinetics; Light; Microfluidics - instrumentation; Microfluidics - methods; Miniaturization; Oligonucleotide Array Sequence Analysis - instrumentation; Oligonucleotide Array Sequence Analysis - methods; Physical Sciences
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.0506848103

Although advances in microfluidic technology have enabled increasingly sophisticated biosensing and bioassay operations to be performed at the microscale, many of these applications employ such small amounts of charged biomolecules (DNA, proteins, and peptides) that they must first be preconcentrated to a detectable level. Efficient strategies for precisely handling minute quantities of biomolecules in microchannel geometries are critically needed; however, it has proven challenging to achieve simultaneous concentration, focusing, and metering capabilities with current-generation sample-injection technology. By using microfluidic chips incorporating arrays of individually addressable microfabricated electrodes, we demonstrate that DNA can be sequentially concentrated, focused into a narrow zone, metered, and injected into an analysis channel. This technique transports charged biomolecules between active electrodes upon application of a small potential difference (1 V) and is capable of achieving orders of magnitude concentration increases within a small device footprint. The collected samples are highly focused, with sample zone size and shape defined solely by electrode geometry.