Sequence-dependent hydrolysis of RNA using modified oligonucleotide splints and RNase H

We found that, in the presence of chimeric oligonucleotides containing complementary deoxyribo- and 2′- O-methylnucleosides, a nonaribonucleotide, [5′- 32 pACUUACCUG, was cleaved specifically upon treatment with RNase H. When 3′m(UG)d(AATG)m(GAC)5′ was used as a hybridization strand, *pACUUACCUG was...

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Bibliographic Details
Published in:	FEBS letters Vol. 215; no. 2; pp. 327 - 330
Main Authors:	Inoue, Hideo, Hayase, Yoji, Iwai, Shigenori, Ohtsuka, Eiko
Format:	Journal Article
Language:	English
Published:	Amsterdam Elsevier B.V 11-05-1987 Elsevier
Subjects:	2′- O-Methyloligonucleotide Analytical, structural and metabolic biochemistry Autoradiography Base Sequence Biological and medical sciences Chimeric oligonucleotide Endoribonucleases Enzymes and enzyme inhibitors Fundamental and applied biological sciences. Psychology Heteroduplex Hydrolases Hydrolysis Nucleic Acid Hybridization Oligonucleotides Ribonuclease H RNA RNA hydrolysis RNase H Site-specific cleavage Heteroduplex 2′- O-Methyloligonucleotide Site-specific cleavage RNase H Chimeric oligonucleotide RNA hydrolysis Hydrolysis RNA Enzyme Reaction mechanism Ribonuclease H DNA RNA hybrid Oligodeoxyribonucleotide
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