Dentine biomodification by sulphonamides pre-treatment: bond strength, proteolytic inhibition, and antimicrobial activity

• Published in: Journal of enzyme inhibition and medicinal chemistry Vol. 38; no. 1; pp. 319 - 329
• Main Authors: Portela, Maristela Barbosa, Barboza, Caroliny Mello, da Silva, Eduardo Moreira, de Moraes, Daniel Clemente, Simão, Renata Antoun, de Souza, Clara Ribeiro, Cardoso, Verônica da Silva, Ferreira-Pereira, Antônio, Vermelho, Alane Beatriz, Supuran, Claudiu T.
• Format: Journal Article
• Language: English
• Published: England Taylor & Francis 01-12-2023; Taylor & Francis Ltd; Taylor & Francis Group
• Subjects: Adhesion; Adhesives; Anti-Infective Agents - pharmacology; Antibacterial activity; Antimicrobial activity; Antimicrobial agents; Biofilms; Bond strength; Carbonic anhydrase inhibitor; Collagen; Collagenase; Composite materials; Dental caries; Dental enamel; Dentin - chemistry; dentine; Enzymes; Gelatinase; Humans; metalloproteinases; Molars; Proteolysis; Research Paper; Spectroscopy, Fourier Transform Infrared; Streptococcus infections; Streptococcus mutans biofilm; Sulfonamides; Sulfonamides - pharmacology; Teeth; metalloproteinases; Streptococcus mutans biofilm; antimicrobial activity; dentine; Carbonic anhydrase inhibitor
• ISSN: 1475-6366; 1475-6374; 1475-6374
• DOI: 10.1080/14756366.2022.2150184

We evaluated the effects of dentine biomodification after pre-treatment with two sulphonamide carbonic anhydrase inhibitors (CAIs) of the N-[4-sulphamoylphenethylcarbamoyl]benzenesulphonamide type, investigating matrix metalloproteases activity, resin-dentine micro tensile bond strength, dentine surface wettability, and antimicrobial activities. Ninety-five sound-extracted human molars were selected for the study. Inhibitory effects were evaluated by gelatinase and collagenase activity tests and collagen degradation FT-IR spectroscopic analysis. Pre-treatment with the two CAIs kept the micro tensile values after 12 months of storage (32.23 ± 5.95) and cariogenic challenge (34.13 ± 2.71) similar to the initial, pre-treatment values (33.56 ± 4.34). A decreased Streptococcus mutans biofilm formation on dentine surfaces and antibacterial activity against planktonic bacteria were observed after CAI treatment. Dentine pre-treatment with sulphonamide CAIs maintained adhesion strength stability, allowed better dentine wettability, maintained matrix collagen, and showed anti-S. mutans activity.