Gene for the Catalytic Subunit of Mouse DNA-Dependent Protein Kinase Maps to the scid Locus

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 92; no. 23; pp. 10792 - 10795
• Main Authors: Miller, Robert D., Hogg, Joanna, Ozaki, Jennifer H., Gell, David, Jackson, Stephen P., Riblet, Roy
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences of the United States of America 07-11-1995; National Acad Sciences; National Academy of Sciences
• Subjects: Animals; Base Sequence; Cell lines; Chromosome Mapping; Chromosomes; Complementary DNA; Crosses, Genetic; DNA; DNA-Activated Protein Kinase; DNA-Binding Proteins; Genes; Genetic loci; Genetic mapping; Genetics; Genomes; Mice; Mice, SCID - genetics; Molecular Sequence Data; Muridae; Mutation; Polymorphism, Genetic; Protein-Serine-Threonine Kinases - genetics; Proteins; Recombination, Genetic; Rodents; Severe combined immunodeficiency; Severe Combined Immunodeficiency - genetics; Somatic cells
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.92.23.10792

The gene encoding the catalytic subunit of DNA-dependent protein kinase (DNA-PKcs) has been proposed recently as a candidate gene for the mouse severe combined immune deficiency (scid) locus. We have used a partial cDNA clone for human DNA-PKcsto map the mouse homologue using a large interspecific backcross panel. We found that the mouse gene for DNA-PKcsdoes not recombine with scid, consistent with the hypothesis that scid is a mutation in the mouse gene for DNA-PKcs.