The Effects of Epidermal Growth Factor/Transforming Growth Factor α on the Expression of Placental Lactogen I and II mRNAs in a Rat Choriocarcinoma Cell Line
Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PL, PUT) suggesting that these proteins have important but different roles. The factors that control this differential gene expression are poorly...
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| Published in: | Endocrine Journal Vol. 45; no. 3; pp. 297 - 306 |
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1998
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| Abstract | Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PL, PUT) suggesting that these proteins have important but different roles. The factors that control this differential gene expression are poorly understood. In this paper we describe experiments which investigate the effects of EGF and TGFα on rPLI and rPLII mRNA levels in the rat choriocarcinoma cell line, Rcho. This cell line has been widely used as a model system for studying genes expressed in the rodent placental giant cell. We find that in these cultures both growth factors produce a two fold increase in endogenous rPLI mRNA levels, and a two fold decrease in rPLII mRNA levels. Using DNA transfection assays we have tested the effects of TGFα on the expression of hybrid rPLI and rPLII 5'flanking/luciferase reporter constructs in Rcho cells. The transfected rPLI/luciferase reporter constructs produce a similar twofold increase in reporter expression to that seen for the endogenous mRNA, suggesting that EGF/TGFa positively regulate rPLI mRNA levels by effects on gene transcription. Unlike the endogenous gene, however, the rPLII/luciferase constructs show a five fold increase in rPLII-directed luciferase expression in the presence of TGFα. The effect of EGF/TGFα on placental rPLII mRNA levels appears to involve negative response element(s) located elsewhere in the gene to those regions tested. |
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| AbstractList | Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PL, PUT) suggesting that these proteins have important but different roles. The factors that control this differential gene expression are poorly understood. In this paper we describe experiments which investigate the effects of EGF and TGFα on rPLI and rPLII mRNA levels in the rat choriocarcinoma cell line, Rcho. This cell line has been widely used as a model system for studying genes expressed in the rodent placental giant cell. We find that in these cultures both growth factors produce a two fold increase in endogenous rPLI mRNA levels, and a two fold decrease in rPLII mRNA levels. Using DNA transfection assays we have tested the effects of TGFα on the expression of hybrid rPLI and rPLII 5'flanking/luciferase reporter constructs in Rcho cells. The transfected rPLI/luciferase reporter constructs produce a similar twofold increase in reporter expression to that seen for the endogenous mRNA, suggesting that EGF/TGFa positively regulate rPLI mRNA levels by effects on gene transcription. Unlike the endogenous gene, however, the rPLII/luciferase constructs show a five fold increase in rPLII-directed luciferase expression in the presence of TGFα. The effect of EGF/TGFα on placental rPLII mRNA levels appears to involve negative response element(s) located elsewhere in the gene to those regions tested. Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PLI, PLII) suggesting that these proteins have important but different roles. The factors that control this differential gene expression are poorly understood. In this paper we describe experiments which investigate the effects of EGF and TGFalpha on rPLI and rPLII mRNA levels in the rat choriocarcinoma cell line, Rcho. This cell line has been widely used as a model system for studying genes expressed in the rodent placental giant cell. We find that in these cultures both growth factors produce a two fold increase in endogenous rPLI mRNA levels, and a two fold decrease in rPLII mRNA levels. Using DNA transfection assays we have tested the effects of TGFalpha on the expression of hybrid rPLI and rPLII 5'flanking/luciferase reporter constructs in Rcho cells. The transfected rPLI/luciferase reporter constructs produce a similar two fold increase in reporter expression to that seen for the endogenous mRNA, suggesting that EGF/TGFalpha positively regulate rPLI mRNA levels by effects on gene transcription. Unlike the endogenous gene, however, the rPLII/ luciferase constructs show a five fold increase in rPLII-directed luciferase expression in the presence of TGFalpha. The effect of EGF/TGFalpha on placental rPLII mRNA levels appears to involve negative response element(s) located elsewhere in the gene to those regions tested. |
| Author | ROBERTSON, MAY C. SUN, YUXIANG DUCKWORTH, MARY LYNN |
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| References | 11). Bissonnette F, Cook C, Geoghegan T, Steffen M, Henry J, Yussman MA, Schultz, G (1992) Transforming growth factor a and epidermal growht factor messenger ribonucleic acid and protein levels in human placenta from early, mid, and late gestation. Am J Obstet Gynecol 166: 192-199. 8). Adamson ED, Meek J (1984) Ontogeny of epidermal growth factor receptors during mouse development. Dev Biol 103: 62-70. 3). Yamaguichi M, Ogren L, Endo H, Thordarson G, Bigsby RM, Talamantes F (1992) Production of mouse placental lactogen-I and placental lactogen-II by the same giant cell. Endocrinology 131: 1595-1206. 25). Maniatis T, Fritsch EF, Sambrook J (1982) Molecular Cloning: A Laboratory Manual. Cold Spring Harbor Laboratories, Cold Spring Harbor, N.T. 1). Soares MJ, Faria TN, Roby KF, Deb S (1991) Pregnancy and the prolactin family of hormones: Coordination of anterior pituitary, uterine and placental expression. Endoc Rev 12: 402-423. 5). Duckworth ML, Schroedter IC, Friesen HG (1990) Cellular localization of rat placental lactogen II and rat prolactin-like proteins A and B by in situ hybridization. Placenta 11: 143-155. 4). Faria TN, Deb S, Kwok SCM, Talamantes F, Soares MJ (1990) Ontogeny of placental lactogen-I and placental lactogen-II expression in the developing rat placenta. Dev Biol 141: 279-291. 9). Smith WC, Talamantes F (1986) Characterization of the mouse palcental epidermal growth factor receptor: Changes in receptor number with day of gestation. Placenta 7: 511-522. 31). Massague J, pandiella A (1993) Membrane-anchored growth factors. Ann Rev Biochem 62: 515-541. 14). Faria TN, Soares MJ (1991) Trophoblast cell differentiation: Establishment, characterization, and modulation of a rat trophoblast cell line expressing members of the placental prolactin family. Endocrinology 129: 2895-2906. 39). Wood SA, Kaye PK (1989) Effects of epidermal growth factor on preimplantation mouse embryos. J Reprod Fert 85: 575-582. 13). Verstuyf A, Sobis H, Goebels J, Fonteyn E, Cassiman JJ, Vandeputte M (1990) Establishment and characterization of a continuous in vitro line from a rat choriocarcinoma. Int J Cancer 45: 752-756. 16). Vuille J-C, Cattini PA, Bock ME, Verstuyf A, Schroedter IC, Duckworth ML, Friesen HG (1993) Rat prolactin-like protein A partial gene and promoter structure: Promoter activity in placental and pituitary cells. Mol Cell Biol 96: 91-98. 17). Dai G, Liu B, Szpirer C, Levan G, Kwok SCM, Soares MJ (1996) Prolactin-like protein-C variant: Complementary deoxyribonucleic acid, unique six exon gene structure and trophoblast-cell specific expression. Endocrinology 137: 5009-5019. 35). Mann GB, Fowler KJ, Gabriel A, Nice EC, Williams R, Dunn AR (1993) Mice with a null mutation of the TGFα gene have abnormal skin architecture, wavy hair, curly whiskes and often develop corneal inflammation. Cell 73: 249-261. 29). Yamaguichi M, Ogren L, Kurachi H, Hirota K, Imai T, Talamantes F (1995) Opposite effects of transforming growth factor a and epidermal growth factor on mouse placental lactogen I secretion. Proc Natl Acad Sci USA 9: 2830-2834. 24). Chomczynski P, Sacchi N (1987) Single-step method of RNA isoaltion by acid guanidinium thiocyanatephenol-chloroform extraction. Anal Biochem 162: 156-159. 26). Nickel BE, Kardami E, Cattini PA (1990) Differential expression of human placental growth-hormone variant and chorionic somatomammotropin in culture. Biochem J 267: 653-658. 30). Yamaguichi M, Ogura K, Sakata M, Miyake A (1996) Transforming growth factor-alpha regulates subpopulation of giant cells which secrete mouse placental lactogen-I (mPL-I) and/or mPLII at midpregnancy. J Endocrinol Invest 19: 146-153. 19). Hamlin Gp, Lu X-J, Roby KF, Soares MJ (1994) Recapitulation of the pathway for trophoblast giant cell differentiation in vitro: Stage-specific expression of members of the prolactin gene family. Endocrinology 134: 2390-2396. 15). Shida MM, Ng YK, Soares MJ, Linzer DIH (1993) Trophoblast specific transcription from the mouse placental lactogen-I gene promoter. Mol Endocrinol 7: 181-188. 38). Sibilia M, Wagner EF (1995) Strain-dependent epithelial defects in mice lacking EGF receptor. Science 269: 234-237. 33). Shida MM, Jackson-Grusby LL, Ross SR, Linzer DIH (1992) Placental-specific expression from the mouse placenal lactogen II gene promoter. Proc Natl Acad Sci USA 89: 3864-3868. 18). Orwig KE, Dai G, Rasmussen CA, Soares MJ (1997) Decidual/trophoblast prolactin-related protein: Characterization of gene structure and cell-specific expression. Endocrinology 138: 2491-2500. 22). Nordeen SK (1988) Luciferase reporter vectors for analysis of promoters and enhancers. BioTechniques 6: 454-457. 7). Yamaguichi M, Ogren L, Endo H, Thordarson G, Kensinger R, Talamantes F (1992) Epidermal growth factor stimulates mouse placental lactogen I but inhibits mouse placental lactogen II secretion in vitro. Proc Natl Acad Sci USA 89: 11396-11400. 28). Cross JC, Flannery ML, Blanar MA, Steingrimsson E, Jenkins NA, Copeland NC, Rutter WJ, Werb Z (1995) Hxt encodes a basic helix-loop-helix transcription factor that regulates trophoblast cell development. Development 121: 2513-2523. 36). Luetteke NC, Qiu TH, Peiffer RL, Oliver P, Smithies O, Lee DC (1993) TGFα deficiency results in hair follicle and eye abnormalities in targeted and waved-1 mice. Cell 73: 263-278. 12). Han VKM, Hunter ES, Pratt RM, Zendegui JG, Lee DC (1987) Expression of rat transforming growth factor alpha mRNA during development occurs predominantly in the maternal decidua. Mol Cell Biol 7: 2335-2343. 27). Yamamoto T, Roby KF, Kwok SCM, Soares MJ (1994) Transcriptional activation of cytochrome P450 side chain cleavage enzyme expression during trophoblast cell differnetiation. J Biol Chem 269: 6517-6523. 32). Bonvissuto AC, Lala PK, Kennedy TG, Nygard K, Lee DC, Han VKM (1992) Induciton of transforming growth factor-α gene expression in rat decidua is independent of the conceptus. Biol Reprod 46: 607-616. 6). Shiu PRC, Kelly PA, Friesen HG (1973) Radioreceptor assay for prolactin and otherlactogenic hormones. Science 180: 968-971. 34). Pestell RC, Albanese C, Watanabe G, Johnson J, Eklund N, Lastowiecki P, Jameson JL (1995) Epidermal growth factor and c-Jun act via a common DNA regulatory element to stimulate transcription of the ovine P-450 cholesterol side chain cleavage (CYP11A1) promoter. J Biol Chem 270: 18301-18308. 37). Threadgill DW, Dlugosz AA, Hansen LA, Tennenbaum T, Lichti U, Yee D, LaMantia C,Mourton T, Herrup K, Harris RC, Barnard JA, Yuspa SH, Coffey RJ, Magnuson T (1995) Targeted disruption of mouse EGF receptor effect of genetic background on mutant phenotype. Science 269: 230-238. 40). Shah P, Sun Y, Sepirer C, Duckworth ML (1998) Rat placental lactogen II gene: Characterization of gene structure and placental-specific expression. Endocrinology 139: 967-973. 2). Duckworth ML, Robertson MC, Schroedter IC, Szpirer C, Friesen HG (1993) Molecular genetics and biology of the rat placental prolactin family. In: Soares MJ, Handwerger S, Talamantes F (eds) Trophoblast Cells: Pathways for Maternal-Emryonic Communication. Serono Symposia, USA, Springer-Verlag, New York Inc,169-190. 20). Robertson MC, Croze F, Schrodter IC, Friesen HG (1990) Molecular cloning and expression of rat placental lactogen-I complementary deoxyribonucleic acid. Endocrinology 127: 702-710. 21). Duckworh M, Kirk KL, Friesen HG (1986) Isolation and identification of a cDNA clone of rat placental lactogen II. J Biol Chem 261: 10871-10878. 10). Paria BC, Das SK, Andrews GK, Dey SK (1993) Expression of the epidermal growth factor receptor gene is regulated in mouse blastocysts during delayed implantation. Proc Natl Acad Sci USA 90: 55-59. 23). Cattini PA, Eberhardt NL (1987) Regulated expression of chimaeric genes containing the 5' flanking regions of human growth hormone-related genes in transicently transfected rat anterior pituitary tumour cells. Nucleic Acids Res 15: 1297-1309. |
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| Snippet | Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PL,... Pregnancy in the rat and mouse is characterized by a developmental switch in expression at midgestation between the related placental lactogens I and II (PLI,... |
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| SubjectTerms | Animals Choriocarcinoma - drug therapy Choriocarcinoma - metabolism EGF/TGFα Epidermal Growth Factor - pharmacology Gene Expression Regulation, Neoplastic - physiology Gene regulation Luciferases - biosynthesis Placental Lactogen - genetics Rat placental lactogens Rats RNA, Messenger - biosynthesis Transforming Growth Factor alpha - pharmacology Tumor Cells, Cultured |
| Title | The Effects of Epidermal Growth Factor/Transforming Growth Factor α on the Expression of Placental Lactogen I and II mRNAs in a Rat Choriocarcinoma Cell Line |
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