Regulation of ciliary polarity by the APC/C

Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report tha...

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Published in:	Proceedings of the National Academy of Sciences - PNAS Vol. 106; no. 42; pp. 17799 - 17804
Main Authors:	Ganner, Athina, Lienkamp, Soeren, Schäfer, Tobias, Romaker, Daniel, Wegierski, Tomasz, Park, Tae Joo, Spreitzer, Stefan, Simons, Matias, Gloy, Joachim, Kim, Emily, Wallingford, John B, Walz, Gerd
Format:	Journal Article
Language:	English
Published:	United States National Academy of Sciences 20-10-2009 National Acad Sciences
Series:	From the Cover
Subjects:	Adaptor Proteins, Signal Transducing - chemistry Adaptor Proteins, Signal Transducing - genetics Adaptor Proteins, Signal Transducing - metabolism Amino Acid Sequence Anaphase-Promoting Complex-Cyclosome Animals Animals, Genetically Modified Antibodies Apc2 Subunit, Anaphase-Promoting Complex-Cyclosome Base Sequence Biological Sciences Cell Line Cell Polarity - physiology Cells Cilia Cilia - physiology Conserved Sequence Dishevelled Proteins Drosophila Embryos Epidermal Cells Epidermis Epithelial cells Female Frogs Gene expression regulation Gene Targeting Humans Mice Oligodeoxyribonucleotides, Antisense - genetics Phosphoproteins - chemistry Phosphoproteins - genetics Phosphoproteins - metabolism Recombinant Proteins - genetics Recombinant Proteins - metabolism Sequence Homology, Amino Acid Signal Transduction - physiology Transfection Ubiquitin-Protein Ligase Complexes - deficiency Ubiquitin-Protein Ligase Complexes - genetics Ubiquitin-Protein Ligase Complexes - physiology Ubiquitins Wnt Proteins - metabolism Xenopus laevis - embryology Xenopus laevis - genetics Xenopus laevis - physiology Xenopus Proteins - deficiency Xenopus Proteins - genetics Xenopus Proteins - physiology
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Summary:	Planar cell polarity signaling controls a variety of polarized cell behaviors. In multiciliated Xenopus epidermal cells, recruitment of Dishevelled (Dvl) to the basal body and its localization to the center of the ciliary rootlet are required to correctly position the motile cilia. We now report that the anaphase-promoting complex (APC/C) recognizes a D-box motif of Dvl and ubiquitylates Dvl on a highly conserved lysine residue. Inhibition of APC/C function by knockdown of the ANAPC2 subunit disrupts the polarity of motile cilia and alters the directionality of the fluid movement along the epidermis of the Xenopus embryo. Our results suggest that the APC/C activity enables cilia to correctly polarize in Xenopus epidermal cells.
Bibliography:	ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 Edited by Kathryn V. Anderson, Sloan–Kettering Institute, New York, NY, and approved August 31, 2009 Author contributions: A.G., S.L., T.S., M.S., J.G., and G.W. designed research; A.G., S.L., T.S., D.R., T.W., T.J.P., S.S., and M.S. performed research; A.G., S.L., T.S., T.W., T.J.P., J.G., J.B.W., and G.W. analyzed data; and E.K. and G.W. wrote the paper. 1A.G., S.L., and T.S. contributed equally to this work.
ISSN:	0027-8424 1091-6490
DOI:	10.1073/pnas.0909465106