Generation of neuronal-like cells from umbilical cord blood-derived mesenchymal stem cells of a RFP-transgenic cloned cat

• Published in: Journal of Veterinary Medical Science Vol. 70; no. 7; pp. 723 - 726
• Main Authors: Jin, G.Z.(Gyeongsang National Univ., Jinju (Korea R.)), Yin, X.J, Yu, X.F, Cho, S.J, Choi, E.G, Lee, Y.S, Jeon, J.T, Yee, S.T, Kong, I.K
• Format: Journal Article
• Language: English
• Published: Japan JAPANESE SOCIETY OF VETERINARY SCIENCE 01-07-2008; Japan Science and Technology Agency
• Subjects: ANIMAL TRANSGENIQUE; ANIMALES TRANSGENICOS; Animals; Animals, Genetically Modified; CATS; Cats - blood; CELL COUNTING; CELL DIFFERENTIATION; Cell Differentiation - physiology; CHAT; CLONE; CLONES; CONTEO DE CELULAS; DIFERENCIACION CELULAR; DIFFERENCIATION CELLULAIRE; differentiation; Fetal Blood - cytology; Flow Cytometry - veterinary; GATO; Luminescent Proteins - chemistry; Luminescent Proteins - genetics; mesenchymal stem cell; Mesenchymal Stem Cells - cytology; Microscopy, Fluorescence - veterinary; NERVOUS SYSTEM; neuronal-like cell; Neurons - cytology; NUMERATION CELLULAIRE; Red Fluorescent Protein; SISTEMA NERVIOSO; SYSTEME NERVEUX; TRANSGENIC ANIMALS
• ISSN: 0916-7250; 1347-7439
• DOI: 10.1292/jvms.70.723

Umbilical cord blood (UCB)-derived mesenchymal stem cells (MSCs) are multipotent adult stem cells, which can differentiate into cells of connective tissue and neural lineages. This study investigated the potential for neuronal differentiation of red fluorescent protein (RFP)-transgenic cat UCB-derived MSCs. The cells were cultured in pre-induction medium for 24 hr and in neuronal-induction medium for 72 hr. Immunofluorescent staining showed that 6.85% of the total cells were beta III-tubulin-positive, 3.37% were neurofilament light (NF-L)-positive and 7.04% were neurofilament medium (NF-M)-positive. A beta III-tubulin band was detected by western blot analysis. Our results demonstrate that RFP-transgenic UCB-derived MSCs can be differentiated into neuronal cells in vitro. Thus, RFP-transgenic MSCs could provide alternative tracing material for stem cell transplantation.