Extensive personal human gut microbiota culture collections characterized and manipulated in gnotobiotic mice

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 108; no. 15; pp. 6252 - 6257
• Main Authors: Goodman, Andrew L, Kallstrom, George, Faith, Jeremiah J, Reyes, Alejandro, Moore, Aimee, Dantas, Gautam, Gordon, Jeffrey I
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 12-04-2011; National Acad Sciences
• Series: From the Cover
• Subjects: animal husbandry; Animals; Bacteria; Bacteria - classification; Bacteria - isolation & purification; bacterial communities; Biogeography; Biological Sciences; Cell culture; Cell Culture Techniques; Colonization; Communities; Cultural institutions; Culture collections; Diet; Diets; digestive system; Digestive tract; Donors; Feces - microbiology; Gastrointestinal Tract - microbiology; Genomics; Genotype & phenotype; Germ free animals; Germ-Free Life; Gnotobiotics; Humans; Husbandry; intestinal microorganisms; Metagenome; metagenomics; Mice; Microbiota; phenotype; Phylogeny; Physiology; Rodents; Sequencing; Taxa; Taxonomy
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.1102938108

The proportion of the human gut bacterial community that is recalcitrant to culture remains poorly defined. In this report, we combine high-throughput anaerobic culturing techniques with gnotobiotic animal husbandry and metagenomics to show that the human fecal microbiota consists largely of taxa and predicted functions that are represented in its readily cultured members. When transplanted into gnotobiotic mice, complete and cultured communities exhibit similar colonization dynamics, biogeographical distribution, and responses to dietary perturbations. Moreover, gnotobiotic mice can be used to shape these personalized culture collections to enrich for taxa suited to specific diets. We also demonstrate that thousands of isolates from a single donor can be clonally archived and taxonomically mapped in multiwell format to create personalized microbiota collections. Retrieving components of a microbiota that have coexisted in single donors who have physiologic or disease phenotypes of interest and reuniting them in various combinations in gnotobiotic mice should facilitate preclinical studies designed to determine the degree to which tractable bacterial taxa are able to transmit donor traits or influence host biology.