Dynamic Assembly of MinD on Phospholipid Vesicles Regulated by ATP and MinE

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 99; no. 10; pp. 6761 - 6766
• Main Authors: Hu, Zonglin, Gogol, Edward P., Lutkenhaus, Joe
• Format: Journal Article
• Language: English
• Published: United States National Academy of Sciences 14-05-2002; National Acad Sciences; The National Academy of Sciences
• Subjects: Adenosine triphosphatases; Adenosine Triphosphatases - genetics; Adenosine Triphosphatases - metabolism; Adenosine Triphosphate - metabolism; Bacteria; Bacterial Proteins - metabolism; Biological Sciences; Cell Cycle Proteins; Cellular biology; Escherichia coli - genetics; Escherichia coli - metabolism; Escherichia coli - ultrastructure; Escherichia coli Proteins; Gene expression regulation; Hydrolysis; Light scattering; Mental concentration; Molecules; Nucleotides; Oscillation; Phospholipids; Phospholipids - metabolism; Plasmids; Polymers; Recombinant Fusion Proteins - genetics; Recombinant Fusion Proteins - metabolism; Studies
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.102059099

Selection of the division site in Escherichia coli is regulated by the min system and requires the rapid oscillation of MinD between the two halves of the cell under the control of MinE. In this study we have further investigated the molecular basis for this oscillation by examining the interaction of MinD with phospholipid vesicles. We found that MinD bound to phospholipid vesicles in the presence of ATP and, upon binding, assembled into a well-ordered helical array that deformed the vesicles into tubes. Stimulation of the MinD ATPase by addition of MinE led to disassembly of the tubes and the release of MinD from the vesicles. It is proposed that this MinE-regulated dynamic assembly of MinD underlies MinD oscillation.