Development of a High-Sensitivity Quantitation Method for Arginine Vasopressin by High-Performance Liquid Chromatography Tandem Mass Spectrometry, and Comparison with Quantitative Values by Radioimmunoassay

• Published in: Analytical Sciences Vol. 32; no. 2; pp. 153 - 159
• Main Authors: TSUKAZAKI, Yasuko, SENDA, Naoto, KUBO, Kinya, YAMADA, Shigeru, KUGOH, Hiroyuki, KAZUKI, Yasuhiro, OSHIMURA, Mitsuo
• Format: Journal Article
• Language: English
• Published: Singapore The Japan Society for Analytical Chemistry 2016; Springer Nature Singapore; Japan Science and Technology Agency
• Subjects: accuracy; Analytical Chemistry; analytical sensitivity; analytical specificity; Arginine vasopressin; Arginine Vasopressin - analysis; Arginine Vasopressin - blood; Calibration; Chemistry; Chromatography, High Pressure Liquid - methods; Chromatography, Ion Exchange - methods; electrode; Electrodes; Extraction; High performance liquid chromatography; Human; Humans; liquid chromatography; Markers; Mass spectrometry; Mathematical analysis; matrix effect; Methylene Chloride - chemistry; Parents; plasma; Quality Control; Radioimmunoassay; Radioimmunoassay - methods; Reproducibility of Results; Tandem Mass Spectrometry - methods; electrode; radioimmunoassay; analytical specificity; mass spectrometry; liquid chromatography; accuracy; matrix effect; analytical sensitivity; Arginine vasopressin; plasma
• ISSN: 0910-6340; 1348-2246
• DOI: 10.2116/analsci.32.153

Human plasma arginine vasopressin (AVP) levels serve as a clinically relevant marker of diabetes and related syndromes. We developed a highly sensitive method for measuring human plasma AVP using high-performance liquid chromatography tandem mass spectrometry. AVP was extracted from human plasma using a weak-cation solid-phase extraction plate, and separated on a wide-bore octadecyl reverse-phase column. AVP was quantified in ion-transition experiments utilizing a product ion (m/z 328.3) derived from its parent ion (m/z 542.8). The sensitivity was enhanced using 0.02% dichloromethane as a mobile-phase additive. The lower limit of quantitation was 0.200 pmol/L. The extraction recovery ranged from 70.2 ± 7.2 to 73.3 ± 6.2% (mean ± SD), and the matrix effect ranged from 1.1 – 1.9%. Quality-testing samples revealed interday/intraday accuracy and precision ranging over 0.9 – 3% and –0.3 – 2%, respectively, which included the endogenous baseline. Our results correlated well with radioimmunoassay results using 22 human volunteer plasma samples.