The essential role of toll like receptor-4 in the control of Aggregatibacter actinomycetemcomitans infection in mice

• Published in: Journal of clinical periodontology Vol. 37; no. 3; pp. 248 - 254
• Main Authors: Lima, Hayana Ramos, Gelani, Valéria, Fernandes, Ana Paula, Gasparoto, Thaís Helena, Torres, Sérgio Aparecido, Santos, Carlos Ferreira, Garlet, Gustavo Pompermaier, Da Silva, João Santana, Campanelli, Ana Paula
• Format: Journal Article
• Language: English
• Published: Oxford, UK Blackwell Publishing Ltd 01-03-2010; Blackwell
• Subjects: Actinobacillus Infections - complications; Actinobacillus Infections - immunology; Actinobacillus Infections - microbiology; Aggregatibacter actinomycetemcomitans - immunology; Aggregatibacter actinomycetemcomitans - pathogenicity; Alveolar Bone Loss - etiology; Alveolar Bone Loss - immunology; Alveolar Bone Loss - microbiology; Animals; Biological and medical sciences; Dentistry; Facial bones, jaws, teeth, parodontium: diseases, semeiology; gingival IL-1β; gingival myeloperoxidase; gingival TNF-α; Interleukin-1beta - metabolism; Male; Medical sciences; Mice; Mice, Inbred C3H; Mice, Knockout; Non tumoral diseases; Otorhinolaryngology. Stomatology; periodontal disease; Periodontitis - etiology; Periodontitis - immunology; Periodontitis - microbiology; Peroxidase - metabolism; Toll-Like Receptor 4 - immunology; Toll-Like Receptor 4 - metabolism; Tumor Necrosis Factor-alpha - metabolism; Enzyme; Stomatology; Cytokine; Rodentia; Interleukin 1β; Toll like receptor; Dentistry; gingival TNF-α; gingival IL-1β; Infection; Vertebrata; Periodontal disease; gingival myeloperoxidase; Mammalia; Treatment; Peroxidases; Mouse; Animal; Peroxidase; Oxidoreductases; Gingiva; Tumor necrosis factor α
• ISSN: 0303-6979; 1600-051X
• DOI: 10.1111/j.1600-051X.2009.01531.x

Lima HR, Gelani V, Fernandes AP, Gasparoto TH, Torres SA, Santos CF, Garlet GP, da Silva JS, Campanelli AP. The essential role of toll like receptor‐4 in the control of Aggregatibacter actinomycetemcomitans infection in mice. J Clin Periodontol 2010; 37: 248–254. doi: 10.1111/j.1600‐051X.2009.01531.x.
Objective: Aggregatibacter actinomycetemcomitans is an oral Gram‐negative bacterium that contributes to periodontitis progression. Isolated antigens from A. actinomycetemcomitans could be activating innate immune cells through Toll‐like receptors (TLRs). In this study, we evaluated the role of TLR4 in the control of A. actinomycetemcomitans infection.
Material and Methods: We examined the mechanisms that modulate the outcome of A. actinomycetemcomitans‐induced periodontal disease in TLR4−/− mice. The production of cytokines was evaluated by ELISA. The bacterial load was determined by counting the number of colony‐forming units per gram of tissue.
Results: The results showed that TLR4‐deficient mice developed less severe periodontitis after A. actinomycetemcomitans infection, characterized by significantly lower bone loss and inflammatory cell migration to periodontal tissues. However, the absence of TLR4 facilitated the A. actinomycetemcomitans dissemination. Myeloperoxidase activity was diminished in the periodontal tissue of TLR4−/− mice. We observed a significant reduction in the production of tumour necrosis factor‐alpha (TNF‐α) and interleukin (IL)‐1β in the periodontal tissue of TLR4−/− mice.
Conclusion: The results of this study highlighted the role of TLR4 in controlling A. actinomycetemcomitans infection.