Determination of branched beta-cyclodextrin-prostaglandin complexes using electrospray ionization mass spectrometry

• Published in: Bioscience, biotechnology, and biochemistry Vol. 72; no. 8; pp. 2164 - 2169
• Main Authors: Yamane, N.(JCL Bioassay Corp., Nishiwaki, Hyogo (Japan)), Tozuka, Z, Okada, Y, Honda, C, Nishi, Y, Tanimoto, T
• Format: Journal Article
• Language: English
• Published: Tokyo Japan Society for Bioscience, Biotechnology, and Agrochemistry 01-08-2008; Japan Society for Bioscience Biotechnology and Agrochemistry
• Subjects: beta-Cyclodextrins - analysis; beta-Cyclodextrins - chemistry; Biological and medical sciences; branched β-cyclodextrin; electrospray ionization mass spectrometry; ESPECTROMETRIA DE MASAS; FENOBARBITAL; Fundamental and applied biological sciences. Psychology; inclusion complex; INDUSTRIA FARMACEUTICA; INDUSTRIE PHARMACEUTIQUE; ION; IONES; IONS; MASS SPECTROMETRY; Models, Molecular; Molecular Structure; OLIGOSACARIDOS; OLIGOSACCHARIDE; OLIGOSACCHARIDES; PHARMACEUTICAL INDUSTRY; PHENOBARBITAL; prostaglandin; PROSTAGLANDINAS; PROSTAGLANDINE; PROSTAGLANDINS; Prostaglandins - chemistry; SPECTROMETRIE DE MASSE; Spectrometry, Mass, Electrospray Ionization; β-Cyclodextrin; branched β-cyclodextrin; inclusion complex; electrospray ionization mass spectrometry; Prostaglandin; Complexes; Mass spectrometry; Electrospray
• ISSN: 0916-8451; 1347-6947
• DOI: 10.1271/bbb.80233

Mass spectral measurements by electrospray ionization mass spectrometry (ESI-MS) detected the ions of beta-cyclodextrin (betaCD) or branched betaCDs (glucosyl-, galactosyl-, mannosyl- and maltosyl-betaCD)-prostaglandins (PGs: PGAsub(2), PGDsub(2), PGEsub(1), PGEsub(2), PGFsub(2alpha) and PGJsub(2)) complexes, i.e., betaCD-PG complexes, with a host:guest ratio of 1:1 in the negative ion mode. This is the first study to report the ions of branched betaCD-PG complexes using ESI-MS. The inclusion complexes were determined by a flow injection analysis using acetonitrile/water. We could confirm by this method the presence of a betaCD-PGEsub(2) complex with a host:guest ratio of 1:1 in a solution-dissolved pharmaceutical formulation consisting of betaCD-PGEsub(2) (Prostarmonsup(TM) E tablet).