Whole genome-based population biology and epidemiological surveillance of Listeria monocytogenes
Listeria monocytogenes ( Lm ) is a major human foodborne pathogen. Numerous Lm outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. We developed a universally applicable genome-wide stra...
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| Published in: | Nature microbiology Vol. 2; no. 2; p. 16185 |
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| Main Authors: | , , , , , , , , , , , , , , , , , , , , , , , , , , , , , |
| Format: | Journal Article |
| Language: | English |
| Published: |
London
Nature Publishing Group UK
10-10-2016
Nature Publishing Group |
| Subjects: | |
| Online Access: | Get full text |
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| Summary: | Listeria monocytogenes
(
Lm
) is a major human foodborne pathogen. Numerous
Lm
outbreaks have been reported worldwide and associated with a high case fatality rate, reinforcing the need for strongly coordinated surveillance and outbreak control. We developed a universally applicable genome-wide strain genotyping approach and investigated the population diversity of
Lm
using 1,696 isolates from diverse sources and geographical locations. We define, with unprecedented precision, the population structure of
Lm
, demonstrate the occurrence of international circulation of strains and reveal the extent of heterogeneity in virulence and stress resistance genomic features among clinical and food isolates. Using historical isolates, we show that the evolutionary rate of
Lm
from lineage I and lineage II is low (∼2.5 × 10
−7
substitutions per site per year, as inferred from the core genome) and that major sublineages (corresponding to so-called ‘epidemic clones’) are estimated to be at least 50–150 years old. This work demonstrates the urgent need to monitor
Lm
strains at the global level and provides the unified approach needed for global harmonization of
Lm
genome-based typing and population biology.
Phylogenetic analysis defines the population and transmission structure of
Listeria monocytogenes
isolates and creates a global framework for epidemiological surveillance.Phylogenetic analysis defines the population and transmission structure of
Listeria monocytogenes
isolates and creates a global framework for epidemiological surveillance. |
|---|---|
| Bibliography: | ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23 This study was designed by S.B., M.L., P.G.-S. and B.P. Selection of isolates was carried out by E.M.N., C.N., V.C.-F., A.L., A.R., K.G., T.D. and L.S.K. DNA preparation and sequencing was performed by H.B.-D., V.C.-F., A.L., C.T., H.C., S.S., Z.K., J.T.B., A.R., C.N., K.G., M.W. and V.E. PFGE analysis was performed by H.B.-D., V.C.-F., A.L. and A.M. Sequence analysis was carried out by A.M., H.P., T.C., L.S.K., H.C. and J.T.B. Definition of core genome was done by M.M.M., E.P.C.R., M.Touc. Validation and reproducibility of cgMLST loci was performed by A.M., H.P. and E.L. Phylogenetic and clustering analyses were carried out by A.M. and A.C. Online database implementation was done by L.J., A.M. and S.B. Epidemiological data analysis was performed by M.Tour. A.L., A.M., T.D., K.G., E.M.N. and C.T. A.M. and S.B. wrote the manuscript, with contributions and comments from all authors. Author contributions |
| ISSN: | 2058-5276 2058-5276 |
| DOI: | 10.1038/nmicrobiol.2016.185 |