Low-level laser irradiation induces in vitro proliferation of mesenchymal stem cells

• Published in: Einstein (São Paulo, Brazil) Vol. 12; no. 1; pp. 75 - 81
• Main Authors: Barboza, Carlos Augusto Galvão, Ginani, Fernanda, Soares, Diego Moura, Henriques, Aguida Cristina Gomes, Freitas, Roseana de Almeida
• Format: Journal Article
• Language: English
• Published: Brazil Instituto Israelita de Ensino e Pesquisa Albert Einstein 01-01-2014
• Subjects: Adipose Tissue - cytology; Animals; Bone Marrow Cells - cytology; Bone Marrow Cells - radiation effects; Cell Proliferation - radiation effects; Cell Survival - drug effects; Cells, Cultured - radiation effects; Células-tronco; Dose-Response Relationship, Radiation; Humans; Lasers, Semiconductor - therapeutic use; Low-Level Light Therapy - methods; Male; MEDICINE, GENERAL & INTERNAL; Medula óssea; Mesenchymal Stromal Cells - cytology; Mesenchymal Stromal Cells - radiation effects; Mice; Original; Proliferação de células; Radiation Dosage; Reproducibility of Results; Tecido adiposo; Terapia a laser; Time Factors; Cell proliferation; Medula óssea; Adipose tissue; Terapia a laser; Proliferação de células; Tecido adiposo; Stem cells; Bone marrow; Laser therapy; Células-tronco
• ISSN: 1679-4508; 2317-6385; 2317-6385; 1679-4508
• DOI: 10.1590/s1679-45082014ao2824

To evaluate the effect of low-level laser irradiation on the proliferation and possible nuclear morphological changes of mouse mesenchymal stem cells.
Mesenchymal stem cells derived from bone marrow and adipose tissue were submitted to two applications (T0 and T48 hours) of low-level laser irradiation (660 nm; doses of 0.5 and 1.0 J/cm2). The trypan blue assay was used to evaluate cell viability, and growth curves were used to analyze proliferation at zero, 24, 48, and 72 hours. Nuclear alterations were evaluated by staining with DAPI (4'-6-diamidino-2-phenylindole) at 72 hours.
Bone marrow-derived mesenchymal stem cells responded to laser therapy in a dose-dependent manner. Higher cell growth was observed when the cells were irradiated with a dose of 1.0 J/cm2, especially after 24 hours (p<0.01). Adipose-derived mesenchymal stem cells responded better to a dose of 1.0 J/cm2, but higher cell proliferation was observed after 48 hours (p<0.05) and 72 hours (p<0.01). Neither nuclear alterations nor a significant change in cell viability was detected in the studied groups.
Low-level laser irradiation stimulated the proliferation of mouse mesenchymal stem cells without causing nuclear alterations. The biostimulation of mesenchymal stem cells using laser therapy might be an important tool for regenerative therapy and tissue engineering.