Screening Preeclamptic Cord Plasma for Proteins Associated with Decreased Breast Cancer Susceptibility

• Published in: Genomics, proteomics & bioinformatics Vol. 11; no. 6; pp. 335 - 344
• Main Authors: Low, Hoi Pang, Tiwari, Ashutosh, Janjanam, Jagadeesh, Qiu, Li, Chang, Chien-I, Strohsnitter, William C., Norwitz, Errol R., Tam, Sun W., Evans, James E., Green, Karin M., Paulo, Joao A., Lambe, Mats, Hsieh, Chung-Cheng
• Format: Journal Article
• Language: English
• Published: England Elsevier Ltd 01-12-2013; Department of Cancer Biology, University of Massachusetts Medical School, Worcester, MA 01605, USA%Department of Chemistry, Michigan Technological University, Houghton, MI 49931, USA%Department of Obstetrics and Gynecology, Tufts Medical Center, Boston, MA 02111, USA; Elsevier
• Subjects: adults; Biomarkers; blood plasma; Blood Proteins - analysis; breast neoplasms; Breast Neoplasms - blood; Breast Neoplasms - chemistry; Chromatography, Liquid; complement; cytoskeleton; Disease Susceptibility; energy; Female; Fetal Blood - chemistry; Fetal Blood - metabolism; gene expression regulation; hemoglobin; Humans; hypertension; Intrauterine environment; keratin; Mass Spectrometry; Original Research; Pilot Projects; pre-eclampsia; Pre-Eclampsia - blood; Preeclampsia; Pregnancy; Prenatal; progeny; protective effect; protein synthesis; proteinuria; Proteomics; risk; screening; spectroscopy; umbilical cord; 乳腺癌; 先兆; 子痫; 特异性蛋白质; 相关蛋白; 等离子; 色素上皮衍生因子; 超高效液相色谱; Pregnancy; Prenatal; Biomarkers; Mass spectrometry; Preeclampsia; Intrauterine environment
• ISSN: 1672-0229; 2210-3244
• DOI: 10.1016/j.gpb.2013.09.009

Preeclampsia, a complication of pregnancy characterized by hypertension and proteinuria, has been found to reduce the subsequent risk for breast cancer in female offspring. As this pro- tective effect could be due to exposure to preeclampsia-specific proteins during intrauterine life, the proteomic profiles of umbilical cord blood plasma between preeclamptic and normotensive pregnancies were compared. Umbilical cord plasma samples, depleted of 14 abundant proteins, were subjected to proteomic analysis using the quantitative method of nanoACQUITY ultra performance liquid chromatography-mass spectrometry with elevated energy mode of acquisitionE （NanoUPLC-MSE）. Sixty-nine differentially expressed proteins were identified, of which 15 and 6 proteins were only detected in preeclamptic and normotensive pregnancies, respectively.Additionally, expression of 8 proteins （gelsolin, complement C5, keratin type I cytoskeletal 10, pigment epithelium-derived factor, complement factor B, complement component C7, hemoglobin subunit gamma-2 and alpha-fetoprotein） were up-regulated in preeclampsia with a fold change of 1〉 2.0 when compared to normotensive pregnancies. The identification of alpha-fetoprotein in pre- eclamptic umbilical cord blood plasma supported the validity of this screen as alpha-fetoprotein has anti-estrogenic properties and has previously been linked to preeclampsia as well as a reduced breast cancer risk. The findings of this pilot study may provide new insights into the mechanistic link between preeclampsia and potentially reduced breast cancer susceptibility in adult life.