Enhanced Bacterial Protein Expression During Auto-Induction Obtained by Alteration of Lac Repressor Dosage and Medium Composition

The auto‐induction method of protein expression in E. coli is based on diauxic growth resulting from dynamic function of lac operon regulatory elements (lacO and LacI) in mixtures of glucose, glycerol, and lactose. The results show that successful execution of auto‐induction is strongly dependent on...

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Bibliographic Details
Published in:	Biotechnology progress Vol. 23; no. 3; pp. 585 - 598
Main Authors:	Blommel, Paul G., Becker, Katie J., Duvnjak, Petar, Fox, Brian G.
Format:	Journal Article
Language:	English
Published:	USA American Chemical Society 01-05-2007 American Institute of Chemical Engineers
Subjects:	Biological and medical sciences Biotechnology Carbon - metabolism Culture Media - pharmacology Electrophoresis, Polyacrylamide Gel Escherichia coli Escherichia coli - genetics Escherichia coli - growth & development Escherichia coli - metabolism Escherichia coli Proteins - genetics Escherichia coli Proteins - metabolism Fundamental and applied biological sciences. Psychology Gene Expression Regulation, Bacterial - drug effects Green Fluorescent Proteins - genetics Green Fluorescent Proteins - metabolism Lac Operon - genetics Luciferases, Bacterial - genetics Luciferases, Bacterial - metabolism Oxygen - pharmacology Photinus Plasmids - genetics Promoter Regions, Genetic - genetics Induction Bacteria Protein
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