Detection of Clavibacter michiganensis subsp. sepedonicus by AmpliDet RNA, a new technology based on real time monitoring of NASBA amplicons with a molecular beacon

Aims: To develop a procedure for direct detection of viable cells of Clavibacter michiganensis subsp. sepedonicus (Cms), the causal organism of bacterial ring rot in potato, based on AmpliDet RNA, in which amplicons generated by nucleic acid sequence based amplification (NASBA) are monitored in real...

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Bibliographic Details
Published in:	Journal of applied microbiology Vol. 93; no. 5; pp. 840 - 849
Main Authors:	Beckhoven, J.R.C.M. van, Stead, D.E, Wolf, J.M. van der
Format:	Journal Article
Language:	English
Published:	Oxford, UK Blackwell Science Ltd 01-01-2002 Blackwell Science Oxford University Press
Subjects:	Actinomycetales - genetics Actinomycetales - isolation & purification bacterial ring rot Bacteriological methods and techniques used in bacteriology Bacteriology Base Sequence Biological and medical sciences Cell Survival cell viability cell walls Clavibacter michiganensis subsp. sepedonicus Computer Systems detection limit DNA DNA, Bacterial - analysis DNA, Bacterial - classification epidemiology Fundamental and applied biological sciences. Psychology Microbiology Molecular Sequence Data monitoring Nucleic Acid Amplification Techniques nucleotide sequences phenol Plant Research International Polymerase Chain Reaction - methods Polymorphism, Restriction Fragment Length ribosomal RNA risk RNA, Ribosomal, 16S - analysis RNA, Ribosomal, 16S - classification Sensitivity and Specificity Sequence Alignment Solanum tuberosum - microbiology technology Bacteria Actinomycetes Method Detection Clavibacter michiganensis
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