Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae

Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein e...

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Bibliographic Details
Published in:	Scientific reports Vol. 10; no. 1; p. 3244
Main Authors:	Kwiatos, Natalia, Jędrzejczak-Krzepkowska, Marzena, Krzemińska, Agnieszka, Delavari, Azar, Paneth, Piotr, Bielecki, Stanisław
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 24-02-2020 Nature Publishing Group Nature Portfolio
Subjects:	631/114 631/114/469 631/114/663 631/45 631/45/535 631/45/535/1267 631/45/603 631/45/607 631/45/607/1168 631/45/612 631/45/612/1141 631/535 631/535/1267 631/61 631/61/168 631/61/185 631/61/338 631/61/338/469 Coal Enzymes Evolution, Molecular Fulvic acids Fusarium - enzymology Fusarium oxysporum Humanities and Social Sciences Humic Substances - analysis Kinetics Laccase Laccase - genetics Laccase - isolation & purification Laccase - metabolism Lignin Molecular Docking Simulation Molecular Dynamics Simulation multidisciplinary Mutation - genetics Protein Engineering Saccharomyces cerevisiae Saccharomyces cerevisiae - metabolism Science Science (multidisciplinary) Thermodynamics Yeast
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