Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae

Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae

Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein e...

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Published in:Scientific reports Vol. 10; no. 1; p. 3244
Main Authors: Kwiatos, Natalia, Jędrzejczak-Krzepkowska, Marzena, Krzemińska, Agnieszka, Delavari, Azar, Paneth, Piotr, Bielecki, Stanisław
Format: Journal Article
Language:English
Published: London Nature Publishing Group UK 24-02-2020
Nature Publishing Group
Nature Portfolio
Subjects:
631/114
631/114/469
631/114/663
631/45
631/45/535
631/45/535/1267
631/45/603
631/45/607
631/45/607/1168
631/45/612
631/45/612/1141
631/535
631/535/1267
631/61
631/61/168
631/61/185
631/61/338
631/61/338/469
Coal
Enzymes
Evolution, Molecular
Fulvic acids
Fusarium - enzymology
Fusarium oxysporum
Humanities and Social Sciences
Humic Substances - analysis
Kinetics
Laccase
Laccase - genetics
Laccase - isolation & purification
Laccase - metabolism
Lignin
Molecular Docking Simulation
Molecular Dynamics Simulation
multidisciplinary
Mutation - genetics
Protein Engineering
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Science
Science (multidisciplinary)
Thermodynamics
Yeast
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Abstract Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three times lower Km, four times higher kcat and ten times higher catalytic efficiency than the parental enzyme. The strategy for laccase engineering was composed of a combination of random methods with a rational approach based on QM/MM MD studies of the enzyme complex with 2,6-dimethoxyphenol. Laccase mediator system with 2,6-dimethoxyphenol caused fulvic acids release from biosolubilized coal.
AbstractList Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three times lower Km, four times higher kcat and ten times higher catalytic efficiency than the parental enzyme. The strategy for laccase engineering was composed of a combination of random methods with a rational approach based on QM/MM MD studies of the enzyme complex with 2,6-dimethoxyphenol. Laccase mediator system with 2,6-dimethoxyphenol caused fulvic acids release from biosolubilized coal.
Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three times lower Km, four times higher kcat and ten times higher catalytic efficiency than the parental enzyme. The strategy for laccase engineering was composed of a combination of random methods with a rational approach based on QM/MM MD studies of the enzyme complex with 2,6-dimethoxyphenol. Laccase mediator system with 2,6-dimethoxyphenol caused fulvic acids release from biosolubilized coal.
Abstract Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards 2,6-dimethoxyphenol, that could be used as a mediator for lignin modification. A combination of classical culture optimization and protein engineering led to around 30 times higher activity in the culture supernatant. The winner mutant exhibited three times lower Km, four times higher kcat and ten times higher catalytic efficiency than the parental enzyme. The strategy for laccase engineering was composed of a combination of random methods with a rational approach based on QM/MM MD studies of the enzyme complex with 2,6-dimethoxyphenol. Laccase mediator system with 2,6-dimethoxyphenol caused fulvic acids release from biosolubilized coal.
ArticleNumber 3244
Author Jędrzejczak-Krzepkowska, Marzena
Krzemińska, Agnieszka
Bielecki, Stanisław
Paneth, Piotr
Delavari, Azar
Kwiatos, Natalia
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  givenname: Marzena
  surname: Jędrzejczak-Krzepkowska
  fullname: Jędrzejczak-Krzepkowska, Marzena
  organization: Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924
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  givenname: Agnieszka
  surname: Krzemińska
  fullname: Krzemińska, Agnieszka
  organization: Institute of Physics, Lodz University of Technology, Wólczańska 219, 90-924
– sequence: 4
  givenname: Azar
  surname: Delavari
  fullname: Delavari, Azar
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  givenname: Piotr
  surname: Paneth
  fullname: Paneth, Piotr
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  givenname: Stanisław
  surname: Bielecki
  fullname: Bielecki, Stanisław
  email: stanislaw.bielecki@p.lodz.pl
  organization: Institute of Technical Biochemistry, Faculty of Biotechnology and Food Sciences, Lodz University of Technology, Stefanowskiego 4/10, 90-924
BackLink https://www.ncbi.nlm.nih.gov/pubmed/32094483$$D View this record in MEDLINE/PubMed
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Snippet Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards...
Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher reactivity towards...
Abstract Fusarium oxysporum laccase was functionally expressed in Saccharomyces cerevisiae and engineered towards higher expression levels and higher...
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Coal
Enzymes
Evolution, Molecular
Fulvic acids
Fusarium - enzymology
Fusarium oxysporum
Humanities and Social Sciences
Humic Substances - analysis
Kinetics
Laccase
Laccase - genetics
Laccase - isolation & purification
Laccase - metabolism
Lignin
Molecular Docking Simulation
Molecular Dynamics Simulation
multidisciplinary
Mutation - genetics
Protein Engineering
Saccharomyces cerevisiae
Saccharomyces cerevisiae - metabolism
Science
Science (multidisciplinary)
Thermodynamics
Yeast
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Title Evolved Fusarium oxysporum laccase expressed in Saccharomyces cerevisiae
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