Association of snR190 snoRNA chaperone with early pre-60S particles is regulated by the RNA helicase Dbp7 in yeast

• Published in: Nature communications Vol. 12; no. 1; p. 6153
• Main Authors: Jaafar, Mariam, Contreras, Julia, Dominique, Carine, Martín-Villanueva, Sara, Capeyrou, Régine, Vitali, Patrice, Rodríguez-Galán, Olga, Velasco, Carmen, Humbert, Odile, Watkins, Nicholas J., Villalobo, Eduardo, Bohnsack, Katherine E., Bohnsack, Markus T., Henry, Yves, Merhi, Raghida Abou, de la Cruz, Jesús, Henras, Anthony K.
• Format: Journal Article
• Language: English
• Published: London Nature Publishing Group UK 22-10-2021; Nature Publishing Group; Nature Portfolio
• Subjects: 13; 38; 38/70; 38/71; 38/77; 38/90; 45; 631/337/1645/501; 631/337/384/521; 631/45/500; 64; 82/29; 82/83; Assembly; Base Pairing; Biosynthesis; DEAD-box RNA Helicases - genetics; DEAD-box RNA Helicases - metabolism; DNA helicase; Genetics; Humanities and Social Sciences; Life Sciences; Maturation; Molecular Chaperones - genetics; Molecular Chaperones - metabolism; multidisciplinary; Mutation; Nuclear Proteins - genetics; Nuclear Proteins - metabolism; Organelle Biogenesis; Precursors; Proteins; Ribonucleic acid; Ribonucleoproteins (small nucleolar); Ribosomal proteins; Ribosome Subunits, Large, Eukaryotic - chemistry; Ribosome Subunits, Large, Eukaryotic - metabolism; Ribosomes; RNA; RNA Folding; RNA helicase; RNA Precursors - chemistry; RNA Precursors - genetics; RNA Precursors - metabolism; RNA, Ribosomal - chemistry; RNA, Ribosomal - genetics; RNA, Ribosomal - metabolism; RNA, Small Nucleolar - genetics; RNA, Small Nucleolar - metabolism; rRNA; rRNA 25S; Saccharomyces cerevisiae; Saccharomyces cerevisiae Proteins - genetics; Saccharomyces cerevisiae Proteins - metabolism; Science; Science (multidisciplinary); snoRNA; Synthesis; Yeast; Yeasts; RNA; RNA folding; Small RNAs
• ISSN: 2041-1723; 2041-1723
• DOI: 10.1038/s41467-021-26207-w

Synthesis of eukaryotic ribosomes involves the assembly and maturation of precursor particles (pre-ribosomal particles) containing ribosomal RNA (rRNA) precursors, ribosomal proteins (RPs) and a
plethora
of assembly factors (AFs). Formation of the earliest precursors of the 60S ribosomal subunit (pre-60S r-particle) is among the least understood stages of ribosome biogenesis. It involves the Npa1 complex, a protein module suggested to play a key role in the early structuring of the pre-rRNA. Npa1 displays genetic interactions with the DExD-box protein Dbp7 and interacts physically with the snR190 box C/D snoRNA. We show here that snR190 functions as a snoRNA chaperone, which likely cooperates with the Npa1 complex to initiate compaction of the pre-rRNA in early pre-60S r-particles. We further show that Dbp7 regulates the dynamic base-pairing between snR190 and the pre-rRNA within the earliest pre-60S r-particles, thereby participating in structuring the peptidyl transferase center (PTC) of the large ribosomal subunit.
The molecular events underlying the assembly and maturation of the early pre-60S particles during eukaryotic ribosome synthesis are not well understood. Here, the authors combine yeast genetics and biochemical experiments to characterise the functions of two important players of eukaryotic ribosome biogenesis, the box C/D snoRNP snR190 and the helicase Dbp7, which both interact. They show that the snR190 snoRNA acts as a RNA chaperone that assists the structuring of the 25S rRNA during the maturation of early pre-60S particles and that Dbp7 is important for facilitating remodeling events in the peptidyl transferase center region of the 25S rRNAs during the maturation of early pre-60S particles.