Su(Ste) diverged tandem repeats in a Y chromosome of Drosophila melanogaster are transcribed and variously processed

• Published in: Genetics (Austin) Vol. 148; no. 1; pp. 243 - 249
• Main Authors: Kalmykova, A.I. (Russian Academy of Sciences, Moscow), Dobritsa, A.A, Gvozdev, V.A
• Format: Journal Article
• Language: English
• Published: United States Genetics Soc America 01-01-1998; Genetics Society of America
• Subjects: ADN; ALTERNATIVE SPLICING; Animals; ARN MENSAJERO; ARN MESSAGER; Base Sequence; CHROMOSOME; CHROMOSOMES; Clones; COMPLEMENTARY DNA; CROMOSOMAS; DNA; DROSOPHILA MELANOGASTER; Drosophila melanogaster - genetics; Female; GENBANK/L42286; GENBANK/L42287; GENBANK/L42288; GENE; GENES; Genes, Insect - genetics; GENETIC POLYMORPHISM; GENETICA; GENETICS; GENETIQUE; INHIBITOR GENES; Insects; INTRONS; Male; MESSENGER RNA; MOLECULAR MAPPING; MOLECULAR SEQUENCE DATA; MULTIPLE GENES; NUCLEOTIDE SEQUENCE; OPEN READING FRAMES; POLIMORFISMO GENETICO; Polymorphism, Genetic; POLYMORPHISME GENETIQUE; Proteins; Repetitive Sequences, Nucleic Acid - genetics; SECUENCIA NUCLEOTIDICA; Sequence Alignment; SEQUENCE NUCLEOTIDIQUE; STE GENE; STRUCTURAL GENES; SU(STE) GENE; TRANSCRIPCION; TRANSCRIPTION; Transcription, Genetic; Y Chromosome - genetics
• ISSN: 0016-6731; 1943-2631; 1943-2631
• DOI: 10.1093/genetics/148.1.243

We report the organization and transcription of diverged tandemly repeated Y-linked Su(Ste) genes that are considered as suppressors of testis-expressed X-linked-repeated Stellate genes that encode a protein sharing extensive homology with beta-subunit of casein kinase 2. Clustering of restriction variants is confirmed. Size variants of Su(Ste) repeats appeared to be nonhomogeneously distributed among the P1 phage clones. Different ways of Su(Ste) RNA processing because of the appearance of new splice sites and polyadenylation signals were detected. The high extent of homology between Stellate and Su(Ste) repeats suggested a possibility of Stellate suppression by antisense transcription of Su(Ste) elements. The detection of only "sense" Su(Ste) cDNAs in testis cDNA library allows us to reject this proposal. The genomic and cDNA clones are shown to be equally diverged. This indicates widespread rather than restricted transcription capacity of these repeats.