Minimally invasive, longitudinal monitoring of biomaterial-associated inflammation by fluorescence imaging

• Published in: Biomaterials Vol. 32; no. 31; pp. 7785 - 7792
• Main Authors: Selvam, Shivaram, Kundu, Kousik, Templeman, Kellie L, Murthy, Niren, García, Andrés J
• Format: Journal Article
• Language: English
• Published: Netherlands Elsevier Ltd 01-11-2011
• Subjects: Advanced Basic Science; Animals; Biocompatibility; Biocompatible Materials - adverse effects; Dentistry; Dexamethasone - pharmacology; Fluorescence; Foreign body response; Free radical; Imaging, Three-Dimensional - methods; Immunohistochemistry; Implants, Experimental - adverse effects; Indocyanine Green - administration & dosage; Indocyanine Green - pharmacology; Inflammation; Inflammation - etiology; Inflammation - pathology; Lactic Acid - pharmacology; Macrophages - drug effects; Mice; Microspheres; Neutrophils - drug effects; Polyglycolic Acid - pharmacology; Reactive Oxygen Species - metabolism; Spectroscopy, Near-Infrared; Superoxide; Biocompatibility; Foreign body response; Inflammation; Superoxide; Free radical
• ISSN: 0142-9612; 1878-5905
• DOI: 10.1016/j.biomaterials.2011.07.020

Abstract Implant-associated inflammation is a major cause for the reduced performance/lifetime and failure of numerous medical devices. Therefore, the ability to non-invasively and quantitatively monitor implant-associated inflammation is critically important. Here we show that implant-associated inflammation can be imaged via fluorescence imaging using near-infrared hydrocyanine dyes delivered either locally or intravenously in living mice. This imaging strategy allowed quantitative longitudinal monitoring of inflammation by detecting reactive oxygen species (ROS) released by inflammatory cells in response to implanted poly(ethylene terephthalate) (PET) disks or injected poly (lactic-co-glycolic acid) (PLGA) microparticles, and exhibited a strong correlation to conventional analysis of inflammation. Furthermore, modulation of inflammatory responses via controlled release of the anti-inflammatory agent dexamethasone was detected using this sensitive imaging approach. Thus, hydrocyanine-based fluorescence imaging of ROS could serve as a surrogate measure for monitoring implant-associated inflammation as well as evaluating the efficacy of therapeutic approaches to modulate host responses to implanted medical devices.