TFC3: gene encoding the B-block binding subunit of the yeast transcription factor IIIC

• Published in: Proceedings of the National Academy of Sciences - PNAS Vol. 89; no. 21; pp. 10512 - 10516
• Main Authors: Lefebvre, O. (Centre d'Etudes de Saclay, Gif sur Yvette, France), Carles, C, Conesa, C, Swanson, R.N, Bouet, F, Riva, M, Sentenac, A
• Format: Journal Article
• Language: English
• Published: Washington, DC National Academy of Sciences of the United States of America 01-11-1992; National Acad Sciences; National Academy of Sciences
• Subjects: Amino Acid Sequence; ARN DE TRANSFERENCIA; ARN DE TRANSFERT; Base Sequence; binding; Biochemistry; Biological and medical sciences; Chromosomes, Fungal; CODE GENETIQUE; CODIGO GENETICO; Codon; DNA; Epitopes - analysis; Fundamental and applied biological sciences. Psychology; Gels; Genes; Genes, Fungal; Genomics; Hemagglutinin Glycoproteins, Influenza Virus; Hemagglutinins, Viral - genetics; Hemagglutinins, Viral - immunology; Introns; LEVADURA; LEVURE; Macromolecular Substances; Molecular and cellular biology; Molecular genetics; Molecular Sequence Data; Mutagenesis, Site-Directed; NUCLEOTIDE; nucleotide sequence; NUCLEOTIDOS; Oligodeoxyribonucleotides; Oligonucleotides; Orthomyxoviridae - immunology; Plasmids; Polymerase chain reaction; Polymerase Chain Reaction - methods; predictions; Protein Biosynthesis; Proteins; Restriction Mapping; Ribonucleic acid; RNA; RNA, Fungal - genetics; RNA, Fungal - isolation & purification; Saccharomyces cerevisiae; Saccharomyces cerevisiae - genetics; Saccharomyces cerevisiae - metabolism; transcription factor TFIIIC; Transcription Factors - genetics; Transcription Factors - metabolism; Transcription Factors, TFIII; Transcription, Genetic; Transcription. Transcription factor. Splicing. Rna processing; TRANSFERASAS; TRANSFERASE; Yeasts; Yeast; Transcription; Enzyme; Transcription promoter; Primary structure; Subunit; Chromosome 1; Fungi; Regulation(control); Binding capacity; Gene; RNA polymerase 3; Ascomycetes; Molecular cloning; Transcription factor; Saccharomyces cerevisiae; Thallophyta
• ISSN: 0027-8424; 1091-6490
• DOI: 10.1073/pnas.89.21.10512

Yeast transcription factor IIIC (TFIIIC) is a multisubunit protein complex that interacts with two control elements of class III promoters called the A and B blocks. Here we describe the gene encoding the 138-kDa subunit (pi 139), which is involved in B-block binding. From the DNA sequence, the open reading frame, interrupted by an intron with an unusual 3' splice acceptor site, is in agreement with all the microsequencing data for peptides within pi 138. TFC3 is a single-copy gene located on chromosome I; it is essential for cell viability as shown by a gene disruption experiment. Epitope-tagging of the TFC3 gene product and DNA binding experiments are consistent with the presence of one copy of pi 138 in TFIIIC-DNA complexes