Toward a genetic tool development pipeline for host-associated bacteria

Highlights • Bacterial members of various microbiota are culturable but lack genetic tools. • Means to boost DNA delivery by mimicking the host’s DNA methylation pattern. • Predictable gene expression by designing or screening promoter, RBS libraries. • Diverse CRISPR technologies for gene editing,...

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Bibliographic Details
Published in:	Current opinion in microbiology Vol. 38; pp. 156 - 164
Main Authors:	Waller, Matthew C, Bober, Josef R, Nair, Nikhil U, Beisel, Chase L
Format:	Journal Article
Language:	English
Published:	England Elsevier Ltd 01-08-2017
Subjects:	Pathology
Online Access:	Get full text
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Summary:	Highlights • Bacterial members of various microbiota are culturable but lack genetic tools. • Means to boost DNA delivery by mimicking the host’s DNA methylation pattern. • Predictable gene expression by designing or screening promoter, RBS libraries. • Diverse CRISPR technologies for gene editing, repression, high-throughput screens. • Progress toward a pipeline for expediting genetic tool development.
Bibliography:	ObjectType-Article-2 SourceType-Scholarly Journals-1 ObjectType-Feature-3 content type line 23 ObjectType-Review-1
ISSN:	1369-5274 1879-0364
DOI:	10.1016/j.mib.2017.05.006