Labeling native bacterial RNA in live cells

Labeling RNA molecules in their physiological environment is still a technological challenge that should be overcome to study kinetics, localization and protein interactions of these ubiquitous cellular regulators. The only non-invasive method applicable for detecting unmodified RNAs in live cells d...

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Bibliographic Details
Published in:	Cell research Vol. 24; no. 7; pp. 894 - 897
Main Authors:	Toran, Paul, Smolina, Irina, Driscoll, Harry, Ding, Feng, Sun, Yingjie, Cantor, Charles R, Broude, Natalia E
Format:	Journal Article
Language:	English
Published:	London Nature Publishing Group UK 01-07-2014 Nature Publishing Group
Subjects:	631/1647/1407 631/1647/1888/1891 Aptamers, Nucleotide - chemistry Bacteria Biomedical and Life Sciences Cell Biology Escherichia coli - genetics Escherichia coli - metabolism Letter to the Editor Life Sciences RNA RNA Probes RNA, Bacterial Staining and Labeling - methods Transformation, Bacterial 活细胞生理环境细菌荧光蛋白蛋白质相互作用调节剂贴标机
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Summary:	Labeling RNA molecules in their physiological environment is still a technological challenge that should be overcome to study kinetics, localization and protein interactions of these ubiquitous cellular regulators. The only non-invasive method applicable for detecting unmodified RNAs in live cells described so far used two RNA-binding pumilio proteins directly interacting with RNA that triggered complementation of the split fluorescent protein （FP） fused to pumilio proteins .
Bibliography:	31-1568/Q Labeling RNA molecules in their physiological environment is still a technological challenge that should be overcome to study kinetics, localization and protein interactions of these ubiquitous cellular regulators. The only non-invasive method applicable for detecting unmodified RNAs in live cells described so far used two RNA-binding pumilio proteins directly interacting with RNA that triggered complementation of the split fluorescent protein （FP） fused to pumilio proteins . SourceType-Other Sources-1 ObjectType-Article-2 content type line 63 ObjectType-Correspondence-1 ObjectType-Article-1 SourceType-Scholarly Journals-1 ObjectType-Feature-2 content type line 23
ISSN:	1001-0602 1748-7838
DOI:	10.1038/cr.2014.47