Reciprocal regulation of the human sterol regulatory element binding protein (SREBP)-1a promoter by Sp1 and EGR-1 transcription factors

• Published in: FEBS letters Vol. 582; no. 2; pp. 177 - 184
• Main Authors: Fernández-Álvarez, Ana, Tur, Gemma, López-Rodas, Gerardo, Casado, Marta
• Format: Journal Article
• Language: English
• Published: England Elsevier B.V 23-01-2008
• Subjects: Base Sequence; Cell Line; ChIP; Chromatin Immunoprecipitation; early growth response gene 1; Early Growth Response Transcription Factors - physiology; Egr-1; Electrophoretic Mobility Shift Assay; EMSA; Gene expression; HEK293T cells; human embryonic kidney 293T cells; Humans; Molecular Sequence Data; NFκB; nuclear factor kappa B; Promoter; Promoter Regions, Genetic; Reverse Transcriptase Polymerase Chain Reaction; Sequence Homology, Nucleic Acid; Sp1; Sp1 Transcription Factor - physiology; SREBP; SREBP-1a; sterol regulatory element binding protein; Sterol Regulatory Element Binding Protein 1 - genetics; the early growth response 1 protein; Promoter; HEK293T cells; SREBP; Cell line; Sp1; EMSA; egr-1; NF κB; ChIP; Gene expression; SREBP-1a
• ISSN: 0014-5793; 1873-3468
• DOI: 10.1016/j.febslet.2007.11.083

Sterol regulatory element binding protein (SREBP)-1a is a transcription factor that is highly expressed in actively growing cells, and is involved in the biosynthesis of cholesterol, fatty acids and phospholipids. We have mapped the minimal human SREBP-1a promoter region to 75
bp upstream of the translation start site where we discovered a functional role for the 3 GC-boxes containing overlapping sites for the Sp1 and EGR-1 transcription factors. Intact SP1-binding sites are essential for promoter activity, whereas EGR-1 suppresses the transcription of the human SREBP-1a promoter. These results reveal a novel physiologically relevant transcriptional mechanism for the reciprocal regulation of the SREBP-1a expression.