Expression of inducible nitric oxide synthase by stimulated macrophages correlates with their antihistoplasma activity

• Published in: Infection and Immunity Vol. 62; no. 4; pp. 1478 - 1479
• Main Authors: Lane, T.E, Otero, G.C, Wu-Hsieh, B.A, Howard, D.H
• Format: Journal Article
• Language: English
• Published: Washington, DC American Society for Microbiology 01-04-1994
• Subjects: Amino Acid Oxidoreductases - biosynthesis; Animals; Biological and medical sciences; Cell Line; ENZIMAS; ENZYME; Fundamental and applied biological sciences. Psychology; HISTOPLASMA; Histoplasma - immunology; Histoplasma capsulatum; INTERFERON; Interferon-gamma - pharmacology; INTERFERONAS; MACROFAGOS; MACROPHAGE; Macrophages - drug effects; Macrophages - enzymology; Macrophages - immunology; Mice; Microbiology; Mycology; Nitric Oxide Synthase; OXIDOS DE NITROGENO; OXYDE D'AZOTE; Pathogenicity, host-agent relations, miscellaneous strains, epidemiology; RATON; Recombinant Proteins; SOURIS; Immunostimulation; Enzyme; Cytokine; Rodentia; Histoplasma capsulatum; Host agent relation; Biological activity; Nitric-oxide synthase; Fungi; Vertebrata; Cell line; Mammalia; Mouse; Nitrogen monoxide; Fungi Imperfecti; Oxidoreductases; Antibacterial agent; Thallophyta; Gamma interferon; Macrophage
• ISSN: 0019-9567; 1098-5522
• DOI: 10.1128/IAI.62.4.1478-1479.1994

The antihistoplasma activity of recombinant murine gamma interferon (rMuIFN-gamma)-treated macrophages of the RAW 264.7 cell line depends on the generation of nitric oxide NO-) from L-arginine. Macrophages of the P388D(1) cell line treated with rMuIFN-gamma do not produce NO. or inhibit the intracellular growth of Histoplasma capsulatum. NO. is generated by the inducible enzyme nitric oxide synthase (iNOS) formed by stimulated macrophages. Northern (RNA) blot analysis of RAW 264.7 cells revealed the expression of iNOS mRNA after exposure to rMuIFN-gamma. In contrast, rMuIFN-gamma-treated P388D(1) cells did not produce detectable levels of iNOS. These data suggest that the failure of P388D(1) cells to generate NO. and to restrict the intracellular growth of H. capsulatum is due to a lack of expression of iNOS following treatment with rMuIFN-gamma