Diversity, abundance, and host relationships of avian malaria and related haemosporidians in New Mexico pine forests

• Published in: PeerJ (San Francisco, CA) Vol. 5; p. e3700
• Main Authors: Marroquin-Flores, Rosario A, Williamson, Jessie L, Chavez, Andrea N, Bauernfeind, Selina M, Baumann, Matthew J, Gadek, Chauncey R, Johnson, Andrew B, McCullough, Jenna M, Witt, Christopher C, Barrow, Lisa N
• Format: Journal Article
• Language: English
• Published: United States PeerJ. Ltd 17-08-2017; PeerJ, Inc; PeerJ Inc
• Subjects: Abundance; Analysis; Apicomplexa; Biodiversity; Bird communities; Birds; Climate change; Community structure; Coniferous forests; Demography; Diseases; Environmental aspects; Environmental protection; Field study; Haemoproteus; Haplotypes; Health aspects; Host specificity; Host-parasite relationships; Infections; Land use planning; Leucocytozoon; Malaria; Microscopy; Mitochondrial DNA; Mosquitoes; Parasites; Parasitology; Phylogenetics; Pinus ponderosa; Pinyon-juniper woodland; Plasmodium; Polymerase chain reaction; Ponderosa pine forest; Zoology; New Mexico; United States > US; California; Plasmodium; Apicomplexa; Haemosporida; Host-parasite relationships; Birds; Wildlife disease; Haemoproteus; Pinyon-juniper woodland; Ponderosa pine forest; Leucocytozoon
• ISSN: 2167-8359; 2167-8359
• DOI: 10.7717/peerj.3700

Avian malaria and related haemosporidian parasites (genera
,
, and
) affect bird demography, species range limits, and community structure, yet they remain unsurveyed in most bird communities and populations. We conducted a community-level survey of these vector-transmitted parasites in New Mexico, USA, to describe their diversity, abundance, and host associations. We focused on the breeding-bird community in the transition zone between piñon-juniper woodland and ponderosa pine forests (elevational range: 2,150-2,460 m). We screened 186 birds representing 49 species using both standard PCR and microscopy techniques to detect infections of all three avian haemosporidian genera. We detected infections in 68 out of 186 birds (36.6%), the highest proportion of which were infected with
(20.9%), followed by
(13.4%), then
(8.0%). We sequenced mtDNA for 77 infections representing 43 haplotypes (25
, 12
, 6
). When compared to all previously known haplotypes in the MalAvi and GenBank databases, 63% (27) of the haplotypes we recovered were novel. We found evidence for host specificity at the avian clade and species level, but this specificity was variable among parasite genera, in that
and
were each restricted to three avian groups (out of six), while
occurred in all groups except non-passerines. We found striking variation in infection rate among host species, with nearly universal infection among vireos and no infection among nuthatches. Using rarefaction and extrapolation, we estimated the total avian haemosporidian diversity to be 70 haplotypes (95% CI [43-98]); thus, we may have already sampled ∼60% of the diversity of avian haemosporidians in New Mexico pine forests. It is possible that future studies will find higher diversity in microhabitats or host species that are under-sampled or unsampled in the present study. Fortunately, this study is fully extendable via voucher specimens, frozen tissues, blood smears, parasite images, and documentation provided in open-access databases (MalAvi, GenBank, and ARCTOS).