No Influence of Exogenous Hyaluronan on the Behavior of Human Cancer Cells or Endothelial Cell Capillary Formation

• Published in: Journal of food science Vol. 79; no. 7; pp. T1469 - T1475
• Main Authors: Seino, Satoshi, Takeshita, Fumitaka, Asari, Akira, Masuda, Yasunobu, Kunou, Masaaki, Ochiya, Takahiro
• Format: Journal Article
• Language: English
• Published: United States The Institute 01-07-2014; Blackwell Publishing Ltd; Wiley Subscription Services, Inc
• Subjects: Animals; Cancer; Capillarity; Cell Line; Cell Line, Tumor; Cell Movement - drug effects; Cell Proliferation - drug effects; Cells; Endothelial cells; Endothelial Cells - drug effects; extracellular matrix; Extracellular Matrix - metabolism; food safety; Formations; functional food; Functional foods & nutraceuticals; human umbilical vein endothelial cells; Humans; Hyaluronan; Hyaluronic Acid - chemistry; Hyaluronic Acid - pharmacology; Hydroxyapatite; image analysis; immunohistochemistry; In vitro testing; Male; Medical research; metastasis; Mice; Molecular Weight; Neovascularization, Pathologic - chemically induced; Neovascularization, Pathologic - metabolism; oral administration; polysaccharides; Surgical implants; food safety; polysaccharides; cancer; functional food; hyaluronan
• ISSN: 0022-1147; 1750-3841
• DOI: 10.1111/1750-3841.12500

Hyaluronan (HA), a type of glycosaminoglycan used to construct the extracellular matrix, is involved in the proliferation and motility of cells, including cancer cells. The aim of this study was to determine whether exogenous HA has an influence on cancer in vitro and in vivo. High‐molecular‐weight HA (900 kDa) and low‐molecular‐weight HA (10 kDa) were added to several types of cancer cell lines in vitro, and proliferation and invasion were assessed. The effect of HA on capillary formation by human umbilical vein endothelial cells was also analyzed. The results showed that both types of HA had no apparent effect on cellular proliferation, invasion, or capillary formation. In an animal study, the 2 types of HA were orally administered to tumor‐bearing mice at a dosage of 200 mg/kg/d for 4 wk. Analysis using an in vivo imaging system revealed that tumor proliferation and metastasis were not greatly altered by HA administration. Furthermore, CD31 immunohistochemical staining revealed no obvious change in tumor microvessels. Taken together, these results demonstrate that exogenously administered HA has little effect on cancer. This study may support the safety of various forms of HA administration, including oral intake.