Comparison and phylogenetic analysis based on the B2L gene of orf virus from goats and sheep in China during 2009-2011

• Published in: Archives of virology Vol. 159; no. 6; pp. 1475 - 1479
• Main Authors: Zhang, Keshan, Liu, Yongjie, Kong, Hanjin, Shang, Youjun, Liu, Xiangtao
• Format: Journal Article
• Language: English
• Published: Vienna Springer Vienna 01-06-2014; Springer Nature B.V
• Subjects: Animals; Biomedical and Life Sciences; Biomedicine; Brief Report; China; Cluster Analysis; Disease; DNA, Viral - chemistry; DNA, Viral - genetics; Ecthyma, Contagious - epidemiology; Ecthyma, Contagious - virology; Epidemiology; Genomes; Genotype; Goats; Infectious Diseases; Medical Microbiology; Molecular Epidemiology; Molecular Sequence Data; Orf virus; Orf virus - classification; Orf virus - genetics; Orf virus - isolation & purification; Phylogenetics; Phylogeny; Provinces; Sequence Analysis, DNA; Sequence Homology; Sheep; Vaccines; Viral Proteins - genetics; Virology; Zoonoses; China; United States > US; India; Vaccine Strain; Supplementary Material Figure; Attenuate Vaccine Strain; Unique Amino Acid Substitution; Chinese Province
• ISSN: 0304-8608; 1432-8798
• DOI: 10.1007/s00705-013-1946-6

As a zoonotic infectious disease, orf outbreaks have been reported in China in recent years. However, molecular epidemiology analysis has not been performed for Chinese orf virus (ORFV) strains. Here, we have identified 13 ORFVs from goats and sheep in China between 2009 and 2011. Thirty-four complete
B2L
sequences were used to construct a phylogenetic tree to elucidate the molecular epidemiology of ORFV in China. Nucleotide sequences of
B2L
genes of clinical samples and attenuated vaccine strains were aligned and compared. Three genotypes were found by molecular epidemiology analysis. Amino acid substitutions were dispersed among
B2
polypeptides from wild and attenuated ORFV strains.